Low Proliferative Potential and Impaired Angiogenesis of Cultured Rat Kidney Endothelial Cells

David Basile, Pingyu Zeng, Jessica L. Friedrich, Ellen C. Leonard, Mervin Yoder

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Objective: CKD is histologically characterized by interstitial fibrosis, which may be driven by peritubular capillary dropout and hypoxia. Surprisingly, peritubular capillaries have little repair capacity. We sought to establish long-term cultures of rat kidney endothelial cells to investigate their growth regulatory properties. Methods: AKEC or YKEC were isolated using CD31-based isolation techniques and sustained in long-term cultures. Results: Although YKEC grew slightly better than AKEC, both performed poorly compared with endothelial cells of the rat adult PMVEC, PAEC, or HUVEC cells. PMVEC and PAEC contained a large percentage of cells with high colony-forming potential. In contrast, KECs were incapable of forming large colonies and most remained as single nondividing cells. KEC expressed high levels of mRNA for VEGF receptors, but were surprisingly insensitive to VEGF stimulation. KEC did not form branching structures on Matrigel when cultured alone, but in mixed cultures, KEC incorporated into branching structures with PMVEC. Conclusions: These data suggest that the intrinsic growth of rat kidney endothelial cells is limited by unknown mechanisms. The low growth rate may be related to the minimal intrinsic regenerative capacity of renal capillaries.

Original languageEnglish
Pages (from-to)598-609
Number of pages12
JournalMicrocirculation
Volume19
Issue number7
DOIs
StatePublished - Oct 2012

Fingerprint

Endothelial Cells
Kidney
Growth
Vascular Endothelial Growth Factor Receptor
Human Umbilical Vein Endothelial Cells
Vascular Endothelial Growth Factor A
Fibrosis
Messenger RNA
Hypoxia
matrigel

Keywords

  • Angiogenesis
  • CKD
  • Hypoxia
  • Progenitor

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)
  • Molecular Biology
  • Cardiology and Cardiovascular Medicine

Cite this

Low Proliferative Potential and Impaired Angiogenesis of Cultured Rat Kidney Endothelial Cells. / Basile, David; Zeng, Pingyu; Friedrich, Jessica L.; Leonard, Ellen C.; Yoder, Mervin.

In: Microcirculation, Vol. 19, No. 7, 10.2012, p. 598-609.

Research output: Contribution to journalArticle

Basile, David ; Zeng, Pingyu ; Friedrich, Jessica L. ; Leonard, Ellen C. ; Yoder, Mervin. / Low Proliferative Potential and Impaired Angiogenesis of Cultured Rat Kidney Endothelial Cells. In: Microcirculation. 2012 ; Vol. 19, No. 7. pp. 598-609.
@article{ad6a5a22f644419cb4fa15b148e7cd03,
title = "Low Proliferative Potential and Impaired Angiogenesis of Cultured Rat Kidney Endothelial Cells",
abstract = "Objective: CKD is histologically characterized by interstitial fibrosis, which may be driven by peritubular capillary dropout and hypoxia. Surprisingly, peritubular capillaries have little repair capacity. We sought to establish long-term cultures of rat kidney endothelial cells to investigate their growth regulatory properties. Methods: AKEC or YKEC were isolated using CD31-based isolation techniques and sustained in long-term cultures. Results: Although YKEC grew slightly better than AKEC, both performed poorly compared with endothelial cells of the rat adult PMVEC, PAEC, or HUVEC cells. PMVEC and PAEC contained a large percentage of cells with high colony-forming potential. In contrast, KECs were incapable of forming large colonies and most remained as single nondividing cells. KEC expressed high levels of mRNA for VEGF receptors, but were surprisingly insensitive to VEGF stimulation. KEC did not form branching structures on Matrigel when cultured alone, but in mixed cultures, KEC incorporated into branching structures with PMVEC. Conclusions: These data suggest that the intrinsic growth of rat kidney endothelial cells is limited by unknown mechanisms. The low growth rate may be related to the minimal intrinsic regenerative capacity of renal capillaries.",
keywords = "Angiogenesis, CKD, Hypoxia, Progenitor",
author = "David Basile and Pingyu Zeng and Friedrich, {Jessica L.} and Leonard, {Ellen C.} and Mervin Yoder",
year = "2012",
month = "10",
doi = "10.1111/j.1549-8719.2012.00193.x",
language = "English",
volume = "19",
pages = "598--609",
journal = "Microcirculation",
issn = "1073-9688",
publisher = "Wiley-Blackwell",
number = "7",

}

TY - JOUR

T1 - Low Proliferative Potential and Impaired Angiogenesis of Cultured Rat Kidney Endothelial Cells

AU - Basile, David

AU - Zeng, Pingyu

AU - Friedrich, Jessica L.

AU - Leonard, Ellen C.

AU - Yoder, Mervin

PY - 2012/10

Y1 - 2012/10

N2 - Objective: CKD is histologically characterized by interstitial fibrosis, which may be driven by peritubular capillary dropout and hypoxia. Surprisingly, peritubular capillaries have little repair capacity. We sought to establish long-term cultures of rat kidney endothelial cells to investigate their growth regulatory properties. Methods: AKEC or YKEC were isolated using CD31-based isolation techniques and sustained in long-term cultures. Results: Although YKEC grew slightly better than AKEC, both performed poorly compared with endothelial cells of the rat adult PMVEC, PAEC, or HUVEC cells. PMVEC and PAEC contained a large percentage of cells with high colony-forming potential. In contrast, KECs were incapable of forming large colonies and most remained as single nondividing cells. KEC expressed high levels of mRNA for VEGF receptors, but were surprisingly insensitive to VEGF stimulation. KEC did not form branching structures on Matrigel when cultured alone, but in mixed cultures, KEC incorporated into branching structures with PMVEC. Conclusions: These data suggest that the intrinsic growth of rat kidney endothelial cells is limited by unknown mechanisms. The low growth rate may be related to the minimal intrinsic regenerative capacity of renal capillaries.

AB - Objective: CKD is histologically characterized by interstitial fibrosis, which may be driven by peritubular capillary dropout and hypoxia. Surprisingly, peritubular capillaries have little repair capacity. We sought to establish long-term cultures of rat kidney endothelial cells to investigate their growth regulatory properties. Methods: AKEC or YKEC were isolated using CD31-based isolation techniques and sustained in long-term cultures. Results: Although YKEC grew slightly better than AKEC, both performed poorly compared with endothelial cells of the rat adult PMVEC, PAEC, or HUVEC cells. PMVEC and PAEC contained a large percentage of cells with high colony-forming potential. In contrast, KECs were incapable of forming large colonies and most remained as single nondividing cells. KEC expressed high levels of mRNA for VEGF receptors, but were surprisingly insensitive to VEGF stimulation. KEC did not form branching structures on Matrigel when cultured alone, but in mixed cultures, KEC incorporated into branching structures with PMVEC. Conclusions: These data suggest that the intrinsic growth of rat kidney endothelial cells is limited by unknown mechanisms. The low growth rate may be related to the minimal intrinsic regenerative capacity of renal capillaries.

KW - Angiogenesis

KW - CKD

KW - Hypoxia

KW - Progenitor

UR - http://www.scopus.com/inward/record.url?scp=84867378238&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84867378238&partnerID=8YFLogxK

U2 - 10.1111/j.1549-8719.2012.00193.x

DO - 10.1111/j.1549-8719.2012.00193.x

M3 - Article

C2 - 22612333

AN - SCOPUS:84867378238

VL - 19

SP - 598

EP - 609

JO - Microcirculation

JF - Microcirculation

SN - 1073-9688

IS - 7

ER -