Lymphangiogenesis does not occur in breast cancer

Beamon Agarwal, Romil Saxena, Akira Morimiya, Sanjana Mehrotra, Sunil Badve

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

Breast cancer metastasis predominantly occurs via lymphatic vessels. However, the study of lymphatic vessels and lymphangiogenesis has been hampered by lack of specific markers. Recently, antibodies directed against M2A (D2-40), Podoplanin, and Prox-1 that specifically mark lymphatic vessels in paraffin-embedded sections have become available. These were used to study lymphangiogenesis in archival paraffin sections of normal breast (n = 23), fibrocystic disease (n = 7), ductal carcinoma in situ (n = 32), invasive ductal carcinoma (n = 50), and invasive lobular carcinoma (n = 5). In addition, endothelial proliferation in lymphatic vessels was analyzed by dual-color immunohistochemistry with D2-40 and proliferating cell nuclear antigen (PCNA). Expression of D2-40, Prox-1, and Podoplanin was seen in lymphatic vessels but not in blood vessels. Lymphatic vessels were seen in the peritumoral area and as "entrapped" intratumoral vessels adjacent to preexisting normal lobules and ducts. Unlike angiogenesis, there was no increase of lymphatic vessel density in association with neoplastic transformation. On the contrary, a marked reduction in intratumoral lymphatic vessel density was seen in comparison to normal breast tissue, fibrocystic disease, and ductal carcinoma in situ (P = 0.0001). There was an increase in peritumoral lymphatic vessel density as compared with normal breast (P = 0.0001). However, the endothelial cells in the "entrapped" or the peritumoral lymphatic vessels did not show any expression of PCNA indicating minimal or no proliferative activity. This was in contrast to the strong expression seen in adjacent tumor cells and blood vessel endothelial cells. Thus, lymphangiogenesis was not evident when studied by lymphatic vessel density or by lymph vessel endothelial proliferation.

Original languageEnglish
Pages (from-to)1449-1455
Number of pages7
JournalAmerican Journal of Surgical Pathology
Volume29
Issue number11
DOIs
StatePublished - Nov 2005

Fingerprint

Lymphangiogenesis
Lymphatic Vessels
Breast Neoplasms
Naproxen
Carcinoma, Intraductal, Noninfiltrating
Proliferating Cell Nuclear Antigen
Paraffin
Breast
Endothelial Cells
Fibrocystic Breast Disease
Vascular Tissue Neoplasms
Lobular Carcinoma
Ductal Carcinoma
Lymph
Blood Vessels
Color

Keywords

  • Breast cancer
  • D2-40
  • Lymphangiogenesis
  • Lymphatic vessel density
  • Podoplanin
  • Prox-1

ASJC Scopus subject areas

  • Anatomy
  • Pathology and Forensic Medicine

Cite this

Lymphangiogenesis does not occur in breast cancer. / Agarwal, Beamon; Saxena, Romil; Morimiya, Akira; Mehrotra, Sanjana; Badve, Sunil.

In: American Journal of Surgical Pathology, Vol. 29, No. 11, 11.2005, p. 1449-1455.

Research output: Contribution to journalArticle

Agarwal, Beamon ; Saxena, Romil ; Morimiya, Akira ; Mehrotra, Sanjana ; Badve, Sunil. / Lymphangiogenesis does not occur in breast cancer. In: American Journal of Surgical Pathology. 2005 ; Vol. 29, No. 11. pp. 1449-1455.
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