Lysophosphatidic acid modulates the regenerative responses of human gingival fibroblasts and enhances the actions of platelet-derived growth factor

D. Roselyn Cerutis, Andrew Dreyer, Franco Cordini, Timothy P. McVaney, John S. Mattson, Lawrence C. Parrish, Laura Romito, Gene R. Huebner, Mansoor Jabro

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Background: Platelet-derived growth factor (PDGF) has been used to promote healing in many in vitro and in vivo models of periodontal regeneration. PDGF is known to interact extensively with another platelet mediator, lysophosphatidic acid (LPA), to enhance regenerative responses in non-oral systems. PDGF and LPA are both liberated by platelets in the blood clot, which is known to be critical in stabilizing early periodontal wound healing. The purpose of this study was to evaluate the basic interactions of LPA with primary human gingival fibroblasts (GF) alone and with PDGF-BB for promoting GF growth and migration, as well as their effects in an in vitro oral wound-healing model. Methods: GF regenerative responses were measured using 1 and 10 μM LPA in the absence or presence of 1 or 10 ng/ml PDGF-BB. Cell growth was determined using [3H]thymidine incorporation and cell counting. Migration responses were measured using a microchemotaxis chamber. For the in vitro wound-healing experiments, GF were grown to confluence on glass slides, and a 3 mm wide wound was mechanically inflicted. Percent wound fill on days 4, 6, and 9 was analyzed using computer-assisted histomorphometry. Results: GF exhibited proliferative and chemotactic responses to LPA. These responses were synergistic when LPA and PDGF-BB were present together. LPA on its own did not stimulate statistically significant wound fill, but when combined with PDGF-BB, wound fill was equivalent to the 10% serum positive control group by day 6 (5.5-fold of negative control, [P

Original languageEnglish (US)
Pages (from-to)297-305
Number of pages9
JournalJournal of Periodontology
Volume75
Issue number2
DOIs
StatePublished - Feb 2004
Externally publishedYes

Fingerprint

Platelet-Derived Growth Factor
Fibroblasts
Wound Healing
Wounds and Injuries
Blood Platelets
Growth
lysophosphatidic acid
Thymidine
Glass
Regeneration
Thrombosis
Control Groups
platelet-derived growth factor BB
Serum
In Vitro Techniques

Keywords

  • Fibroblasts
  • Gingival
  • Growth factors
  • Lysophospholids
  • Models, biological
  • Periodontal regeneration
  • Platelet-derived
  • Wound healing

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Lysophosphatidic acid modulates the regenerative responses of human gingival fibroblasts and enhances the actions of platelet-derived growth factor. / Cerutis, D. Roselyn; Dreyer, Andrew; Cordini, Franco; McVaney, Timothy P.; Mattson, John S.; Parrish, Lawrence C.; Romito, Laura; Huebner, Gene R.; Jabro, Mansoor.

In: Journal of Periodontology, Vol. 75, No. 2, 02.2004, p. 297-305.

Research output: Contribution to journalArticle

Cerutis, D. Roselyn ; Dreyer, Andrew ; Cordini, Franco ; McVaney, Timothy P. ; Mattson, John S. ; Parrish, Lawrence C. ; Romito, Laura ; Huebner, Gene R. ; Jabro, Mansoor. / Lysophosphatidic acid modulates the regenerative responses of human gingival fibroblasts and enhances the actions of platelet-derived growth factor. In: Journal of Periodontology. 2004 ; Vol. 75, No. 2. pp. 297-305.
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AU - Cerutis, D. Roselyn

AU - Dreyer, Andrew

AU - Cordini, Franco

AU - McVaney, Timothy P.

AU - Mattson, John S.

AU - Parrish, Lawrence C.

AU - Romito, Laura

AU - Huebner, Gene R.

AU - Jabro, Mansoor

PY - 2004/2

Y1 - 2004/2

N2 - Background: Platelet-derived growth factor (PDGF) has been used to promote healing in many in vitro and in vivo models of periodontal regeneration. PDGF is known to interact extensively with another platelet mediator, lysophosphatidic acid (LPA), to enhance regenerative responses in non-oral systems. PDGF and LPA are both liberated by platelets in the blood clot, which is known to be critical in stabilizing early periodontal wound healing. The purpose of this study was to evaluate the basic interactions of LPA with primary human gingival fibroblasts (GF) alone and with PDGF-BB for promoting GF growth and migration, as well as their effects in an in vitro oral wound-healing model. Methods: GF regenerative responses were measured using 1 and 10 μM LPA in the absence or presence of 1 or 10 ng/ml PDGF-BB. Cell growth was determined using [3H]thymidine incorporation and cell counting. Migration responses were measured using a microchemotaxis chamber. For the in vitro wound-healing experiments, GF were grown to confluence on glass slides, and a 3 mm wide wound was mechanically inflicted. Percent wound fill on days 4, 6, and 9 was analyzed using computer-assisted histomorphometry. Results: GF exhibited proliferative and chemotactic responses to LPA. These responses were synergistic when LPA and PDGF-BB were present together. LPA on its own did not stimulate statistically significant wound fill, but when combined with PDGF-BB, wound fill was equivalent to the 10% serum positive control group by day 6 (5.5-fold of negative control, [P

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