Macrophage activity in response to steady-state oxygen and hydrogen peroxide concentration

Henry O. Owegi, Stéphane Egot-Lemaire, Lee R. Waite, Gabi N. Waite

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Macrophages are important players of the immune system to fight infections since they eliminate microorganisms. During this process they often encounter a wide range of oxygen concentrations, from roughly 13% O2 (PO 2 = 760 × 0.13 mmHg) in arterial blood to less than 1% O 2 in inflamed tissue. Macrophages contribute to the elimination of microorganisms by releasing oxygen derivates such as hydrogen peroxide. The objective of this study was to test macrophage activity under various O 2 and H2O2 concentrations such as present during infection. We exposed macrophages to steady-state O2 concentrations between 21% and 1% O2 and steady-state H 2O2 concentrations between 0 and 20 μM using a novel enzymatic system. The system uses glucose oxidase (Gox) and catalase (Cat) in standard, open-system, cell culture vessels. Macrophage activity was determined as change in phagocytosis and as release of antimicrobial H2O 2 into extracellular medium. We show that O2 concentrations below 7% enhance the activity of macrophages of the THP-1 cell line in a dose-dependent way; with doubled activity at 1% O2 compared to 21% O2 conditions. We further show that macrophages are able to function in an environment of high H2O2concentrations and are even stimulated by H2O2 concentrations below 20 μM. The activity of hypoxic macrophages is up to 3-fold enhanced in the presence of H2O2 as compared to the activity triggered by low O 2 conditions alone. Our data show that hypoxia and H 2O2, as present in infectious conditions, strongly enhance macrophage activity. The data further demonstrate the usefulness and versatility of the Gox/Cat system for studies of infectious and inflammatory diseases.

Original languageEnglish
Title of host publication47th Annual Rocky Mountain Bioengineering Symposium and 47th International ISA Biomedical Sciences Instrumentation Symposium 2010
Pages38-43
Number of pages6
Volume480
StatePublished - 2010
Event47th Annual Rocky Mountain Bioengineering Symposium and 47th International ISA Biomedical Sciences Instrumentation Symposium 2010 - Laramie, WY, United States
Duration: Apr 9 2010Apr 11 2010

Other

Other47th Annual Rocky Mountain Bioengineering Symposium and 47th International ISA Biomedical Sciences Instrumentation Symposium 2010
CountryUnited States
CityLaramie, WY
Period4/9/104/11/10

Fingerprint

Macrophages
Hydrogen peroxide
Hydrogen Peroxide
Oxygen
Glucose Oxidase
Glucose oxidase
Microorganisms
Catalase
Immune system
Open systems
Cell culture
Blood
Cells
Tissue

Keywords

  • Catalase
  • Glucose oxidase
  • Hypoxia
  • Matlab model
  • OHO
  • Oxidative burst
  • Phagocytosis
  • THP-1

ASJC Scopus subject areas

  • Bioengineering
  • Biomedical Engineering

Cite this

Owegi, H. O., Egot-Lemaire, S., Waite, L. R., & Waite, G. N. (2010). Macrophage activity in response to steady-state oxygen and hydrogen peroxide concentration. In 47th Annual Rocky Mountain Bioengineering Symposium and 47th International ISA Biomedical Sciences Instrumentation Symposium 2010 (Vol. 480, pp. 38-43)

Macrophage activity in response to steady-state oxygen and hydrogen peroxide concentration. / Owegi, Henry O.; Egot-Lemaire, Stéphane; Waite, Lee R.; Waite, Gabi N.

47th Annual Rocky Mountain Bioengineering Symposium and 47th International ISA Biomedical Sciences Instrumentation Symposium 2010. Vol. 480 2010. p. 38-43.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Owegi, HO, Egot-Lemaire, S, Waite, LR & Waite, GN 2010, Macrophage activity in response to steady-state oxygen and hydrogen peroxide concentration. in 47th Annual Rocky Mountain Bioengineering Symposium and 47th International ISA Biomedical Sciences Instrumentation Symposium 2010. vol. 480, pp. 38-43, 47th Annual Rocky Mountain Bioengineering Symposium and 47th International ISA Biomedical Sciences Instrumentation Symposium 2010, Laramie, WY, United States, 4/9/10.
Owegi HO, Egot-Lemaire S, Waite LR, Waite GN. Macrophage activity in response to steady-state oxygen and hydrogen peroxide concentration. In 47th Annual Rocky Mountain Bioengineering Symposium and 47th International ISA Biomedical Sciences Instrumentation Symposium 2010. Vol. 480. 2010. p. 38-43
Owegi, Henry O. ; Egot-Lemaire, Stéphane ; Waite, Lee R. ; Waite, Gabi N. / Macrophage activity in response to steady-state oxygen and hydrogen peroxide concentration. 47th Annual Rocky Mountain Bioengineering Symposium and 47th International ISA Biomedical Sciences Instrumentation Symposium 2010. Vol. 480 2010. pp. 38-43
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