Malic enzyme gene in chick embryo hepatocytes in culture: Clofibrate regulates responsiveness to triiodothyronine

Thomas L. Carlisle, Cesar Roncero, Catherine El Khadir-Mounier, Debbie C. Thurmond, Alan G. Goodridge

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

In chick embryo hepatocytes, triiodothyronine (T3) causes a 30- to 40- fold increase in malic enzyme activity when added between I and 3 days, but has no effect when added between 5 and 7 days in culture. This transcription- mediated decline in T3 responsiveness is partially reversed by corticosterone (Roncero, C. and A. G. Goodridge. 1992. Arch. Biochem. Biophys. 295: 258- 267). Clofibrate also reversed the decline in responsiveness to T3, and did so in the absence of an increase in binding of T3 to nuclear receptors. The effects of clofibrate and corticosterone were additive, suggesting different mechanisms. The responsiveness of a gene to a specific agent depends on specific regulatory sequences of DNA in that gene. When 5.8 kb of the 5'- flanking DNA of the malic enzyme gene was linked to the chloramphenicol acetyltransferase (CAT) gene and transfected into hepatocytes, T3 stimulated CAT activity. Responsiveness of CAT activity to T3 decreased with time, and this decrease was partially reversed by clofibrate. The T3 responses of cells transfected with various chimeric DNAs that contained T3 response elements (T3REs) of the malic enzyme gene or synthetic consensus T3REs also were increased by clofibrate. The results suggest that clofibrate regulates expression of a metabolite or a protein factor which, in turn, influences function of the T3 receptor.

Original languageEnglish (US)
Pages (from-to)2088-2097
Number of pages10
JournalJournal of Lipid Research
Volume37
Issue number10
StatePublished - Oct 1996
Externally publishedYes

Fingerprint

Clofibrate
Triiodothyronine
Chick Embryo
Cell culture
Hepatocytes
Genes
Chloramphenicol O-Acetyltransferase
Enzymes
Response Elements
Corticosterone
DNA
Synthetic Genes
Thyroid Hormone Receptors
Enzyme activity
Arches
Transcription
Cytoplasmic and Nuclear Receptors
Metabolites
Proteins

Keywords

  • peroxisomal proliferating agents
  • thyroid hormone receptor
  • transcription

ASJC Scopus subject areas

  • Endocrinology

Cite this

Carlisle, T. L., Roncero, C., El Khadir-Mounier, C., Thurmond, D. C., & Goodridge, A. G. (1996). Malic enzyme gene in chick embryo hepatocytes in culture: Clofibrate regulates responsiveness to triiodothyronine. Journal of Lipid Research, 37(10), 2088-2097.

Malic enzyme gene in chick embryo hepatocytes in culture : Clofibrate regulates responsiveness to triiodothyronine. / Carlisle, Thomas L.; Roncero, Cesar; El Khadir-Mounier, Catherine; Thurmond, Debbie C.; Goodridge, Alan G.

In: Journal of Lipid Research, Vol. 37, No. 10, 10.1996, p. 2088-2097.

Research output: Contribution to journalArticle

Carlisle, TL, Roncero, C, El Khadir-Mounier, C, Thurmond, DC & Goodridge, AG 1996, 'Malic enzyme gene in chick embryo hepatocytes in culture: Clofibrate regulates responsiveness to triiodothyronine', Journal of Lipid Research, vol. 37, no. 10, pp. 2088-2097.
Carlisle TL, Roncero C, El Khadir-Mounier C, Thurmond DC, Goodridge AG. Malic enzyme gene in chick embryo hepatocytes in culture: Clofibrate regulates responsiveness to triiodothyronine. Journal of Lipid Research. 1996 Oct;37(10):2088-2097.
Carlisle, Thomas L. ; Roncero, Cesar ; El Khadir-Mounier, Catherine ; Thurmond, Debbie C. ; Goodridge, Alan G. / Malic enzyme gene in chick embryo hepatocytes in culture : Clofibrate regulates responsiveness to triiodothyronine. In: Journal of Lipid Research. 1996 ; Vol. 37, No. 10. pp. 2088-2097.
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