Manganese attenuates secretagogue-mediated phospholipid hydrolysis in AR42J cells

Steven A. Siwik, Murray Korc

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

The effects of cholecystokinin octapeptide (CCK8), bombesin and manganese (Mn2+) on phosphatidylinositol-4,5-bisphosphate (PIP2) hydrolysis were studied in AR42J cells. One-half maximal stimulation of inositol monophosphate (InsP1) accumulation occurred at either 5 nM CCK8 or 5 nM bombesin, and maximal stimulation occurred at 30 nM for each agonist. Mn2+ did not alter basal PIP2 hydrolysis. However, addition of Mn2+ 5 min prior to stimulation with either CCK8 or bombesin for 60 min significantly attenuated [3H]InsP1 accumulation. Following brief periods of incubation with CCK8 (15 sec) Mn2+ significantly reduced inositol tris- and tetrakisphosphate accumulation. These data suggest that Mn2+ may participate in the regulation of CCK8- and bombesin-mediated generation of phosphoinositides.

Original languageEnglish (US)
Pages (from-to)1959-1965
Number of pages7
JournalLife Sciences
Volume45
Issue number21
DOIs
StatePublished - 1989

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ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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