Manganese-stimulated phosphorylation of a rat pancreatic protein

identity with elongation factor 2

Simon A B Knight, William Kohr, Murray Korc

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

To investigate the effect of Mn2+ on pancreatic protein phosphorylation, we incubated rat pancreatic cytosol in Tris buffer (pH 7.5) with [γ-32P]ATP. Analysis using sodium dodecyl sulphate polyacrylamide gel electrophoresis and autoradiography revealed a single protein (p98), with an Mr of 98 000 and pI of 6.4-6.5, which was phosphorylated in a dose-dependent manner by Mn2+. A threshold effect was observed at 35 μM, and maximal effect at 1.1 mM Mn2+. Ca2+ and calmodulin (CaM) did not cause p98 phosphorylation, but Mg2+ (10 mM) caused faint non-specific phosphorylation of p98. Ca2+ (0.03-3 mM) and CaM (1-10 μg/ml) significantly enhanced, whereas trifluoperazine (TFP) and Mg2+ inhibited Mn2+-stimulated p98 phosphorylation. Under the above incubation conditions, Mn2+-stimulated protein phosphorylation of p98 was also observed in isolated pancreatic acini, but not in cytosols from liver or kidney. Partial purification of p98 and amino acid sequencing of the protein band corresponding to p98 indicated complete sequence homology with rat elongation factor 2 (EF-2). Furthermore, the combination of Ca2+, Mg2+ and CaM, which is known to induce the phosphorylation of EF-2, mimicked the actions of Mn2+. Inasmuch as EF-2 is the major substrate for CaM-dependent protein kinase III (CaM-PK III), these studies suggest that in the pancreatic acinar cell Mn2+/CaM protein kinase activity is mediated via CaM-PK III and that Mn2+ participates in the regulation of this enzyme in the pancreas.

Original languageEnglish (US)
Pages (from-to)196-204
Number of pages9
JournalBiochimica et Biophysica Acta - Molecular Cell Research
Volume1092
Issue number2
DOIs
StatePublished - Apr 17 1991
Externally publishedYes

Fingerprint

Peptide Elongation Factor 2
Manganese
Phosphorylation
Calmodulin
Proteins
Cytosol
Elongation Factor 2 Kinase
Trifluoperazine
Calcium-Calmodulin-Dependent Protein Kinases
Tromethamine
Acinar Cells
Protein Sequence Analysis
Sequence Homology
Autoradiography
Sodium Dodecyl Sulfate
Protein Kinases
Polyacrylamide Gel Electrophoresis
Pancreas
Adenosine Triphosphate
Kidney

Keywords

  • Calmodulin
  • Elongation factor 2
  • Manganese
  • Pancreas
  • Phosphorylation

ASJC Scopus subject areas

  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Manganese-stimulated phosphorylation of a rat pancreatic protein : identity with elongation factor 2. / Knight, Simon A B; Kohr, William; Korc, Murray.

In: Biochimica et Biophysica Acta - Molecular Cell Research, Vol. 1092, No. 2, 17.04.1991, p. 196-204.

Research output: Contribution to journalArticle

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abstract = "To investigate the effect of Mn2+ on pancreatic protein phosphorylation, we incubated rat pancreatic cytosol in Tris buffer (pH 7.5) with [γ-32P]ATP. Analysis using sodium dodecyl sulphate polyacrylamide gel electrophoresis and autoradiography revealed a single protein (p98), with an Mr of 98 000 and pI of 6.4-6.5, which was phosphorylated in a dose-dependent manner by Mn2+. A threshold effect was observed at 35 μM, and maximal effect at 1.1 mM Mn2+. Ca2+ and calmodulin (CaM) did not cause p98 phosphorylation, but Mg2+ (10 mM) caused faint non-specific phosphorylation of p98. Ca2+ (0.03-3 mM) and CaM (1-10 μg/ml) significantly enhanced, whereas trifluoperazine (TFP) and Mg2+ inhibited Mn2+-stimulated p98 phosphorylation. Under the above incubation conditions, Mn2+-stimulated protein phosphorylation of p98 was also observed in isolated pancreatic acini, but not in cytosols from liver or kidney. Partial purification of p98 and amino acid sequencing of the protein band corresponding to p98 indicated complete sequence homology with rat elongation factor 2 (EF-2). Furthermore, the combination of Ca2+, Mg2+ and CaM, which is known to induce the phosphorylation of EF-2, mimicked the actions of Mn2+. Inasmuch as EF-2 is the major substrate for CaM-dependent protein kinase III (CaM-PK III), these studies suggest that in the pancreatic acinar cell Mn2+/CaM protein kinase activity is mediated via CaM-PK III and that Mn2+ participates in the regulation of this enzyme in the pancreas.",
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N2 - To investigate the effect of Mn2+ on pancreatic protein phosphorylation, we incubated rat pancreatic cytosol in Tris buffer (pH 7.5) with [γ-32P]ATP. Analysis using sodium dodecyl sulphate polyacrylamide gel electrophoresis and autoradiography revealed a single protein (p98), with an Mr of 98 000 and pI of 6.4-6.5, which was phosphorylated in a dose-dependent manner by Mn2+. A threshold effect was observed at 35 μM, and maximal effect at 1.1 mM Mn2+. Ca2+ and calmodulin (CaM) did not cause p98 phosphorylation, but Mg2+ (10 mM) caused faint non-specific phosphorylation of p98. Ca2+ (0.03-3 mM) and CaM (1-10 μg/ml) significantly enhanced, whereas trifluoperazine (TFP) and Mg2+ inhibited Mn2+-stimulated p98 phosphorylation. Under the above incubation conditions, Mn2+-stimulated protein phosphorylation of p98 was also observed in isolated pancreatic acini, but not in cytosols from liver or kidney. Partial purification of p98 and amino acid sequencing of the protein band corresponding to p98 indicated complete sequence homology with rat elongation factor 2 (EF-2). Furthermore, the combination of Ca2+, Mg2+ and CaM, which is known to induce the phosphorylation of EF-2, mimicked the actions of Mn2+. Inasmuch as EF-2 is the major substrate for CaM-dependent protein kinase III (CaM-PK III), these studies suggest that in the pancreatic acinar cell Mn2+/CaM protein kinase activity is mediated via CaM-PK III and that Mn2+ participates in the regulation of this enzyme in the pancreas.

AB - To investigate the effect of Mn2+ on pancreatic protein phosphorylation, we incubated rat pancreatic cytosol in Tris buffer (pH 7.5) with [γ-32P]ATP. Analysis using sodium dodecyl sulphate polyacrylamide gel electrophoresis and autoradiography revealed a single protein (p98), with an Mr of 98 000 and pI of 6.4-6.5, which was phosphorylated in a dose-dependent manner by Mn2+. A threshold effect was observed at 35 μM, and maximal effect at 1.1 mM Mn2+. Ca2+ and calmodulin (CaM) did not cause p98 phosphorylation, but Mg2+ (10 mM) caused faint non-specific phosphorylation of p98. Ca2+ (0.03-3 mM) and CaM (1-10 μg/ml) significantly enhanced, whereas trifluoperazine (TFP) and Mg2+ inhibited Mn2+-stimulated p98 phosphorylation. Under the above incubation conditions, Mn2+-stimulated protein phosphorylation of p98 was also observed in isolated pancreatic acini, but not in cytosols from liver or kidney. Partial purification of p98 and amino acid sequencing of the protein band corresponding to p98 indicated complete sequence homology with rat elongation factor 2 (EF-2). Furthermore, the combination of Ca2+, Mg2+ and CaM, which is known to induce the phosphorylation of EF-2, mimicked the actions of Mn2+. Inasmuch as EF-2 is the major substrate for CaM-dependent protein kinase III (CaM-PK III), these studies suggest that in the pancreatic acinar cell Mn2+/CaM protein kinase activity is mediated via CaM-PK III and that Mn2+ participates in the regulation of this enzyme in the pancreas.

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