MAP kinases: Differential activation following in vivo and ex vivo irradiation

Anirban Kumar Mitra, Rakesh Kumar Singh, Malini Krishna

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Mitogen activated protein kinases (MAPK) play a critical role in controlling cell survival and repopulation following exposure to ionising radiation. Most investigations on these pathways have been done using cultured cells or by ex vivo treatments. The present study was carried out to determine whether the response of MAPKs in mouse lymphocytes differs following in vivo and ex vivo irradiation with 60Co γ-rays. We observed that ex vivo treatment resulted in a very significant decrease in the activated p44/42 and p38 MAPK as compared to in vivo. However, stress activated protein kinase (SAPK) response showed no significant difference between in vivo and ex vivo treatments. These observations point towards the differences in response elicited when the treatment is given in vivo as compared to in vitro. Therefore the findings reported from in vitro or ex vivo treatments should be treated with caution especially if it has to be clinically applied.

Original languageEnglish (US)
Pages (from-to)65-72
Number of pages8
JournalMolecular and Cellular Biochemistry
Volume294
Issue number1-2
DOIs
StatePublished - Jan 2007

Keywords

  • Exvivo
  • In vivo
  • P38MAPK
  • SAPK and lymphocytes
  • p44/42MAPK

ASJC Scopus subject areas

  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

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