Mapping of brain insulin and insulin-like growth factor receptor gene expression by in situ hybridization

Carolyn A. Bondy, Mark A. Bach, Wei Hua Lee

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

The insulin and insulin-like growth factor I (IGF-I) receptors are members of a distinct tyrosine kinase receptor family. In situ hybridization employing ribonucleotide probes was used to compare patterns of insulin and IGF-I receptor gene expression in serial rat brain sections. A striking redundancy in anatomical patterns for insulin and IGF receptor gene expression is seen in many brain regions from olfactory bulb through cerebellum. Both receptor mRNAs are highest and appear to be coexpressed in the neuron-dense granule cell layers of olfactory bulb, dentate gyrus, and cerebellar cortex and pyramidal cell layers of piriform cortex and Ammon's horn, with relatively little hybridization detected in white matter zones. Superimposed on this generalized pattern are distinct local regions of selective enhancement in gene expression for the insulin or IGF-I receptor. Insulin receptor mRNA is more highly concentrated in anterior thalamic and hypothalamic structures which may have access to circulating insulin. IGF-I receptor mRNA is more highly expressed in projection neurons of sensory and cerebellar relay centers where local IGF-I synthesis is also concentrated. The overlap in insulin and IGF-I receptor gene expression found in many brain regions suggests that hybrid insulin-IGF receptors may be expressed in these regions. In summary, the gene expression patterns revealed in this study suggest that the insulin and IGF receptors may be among the most abundant and ubiquitous of the brain tyrosine kinases.

Original languageEnglish (US)
Pages (from-to)240-249
Number of pages10
JournalNeuroprotocols
Volume1
Issue number3
DOIs
StatePublished - 1992
Externally publishedYes

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Brain Mapping
Somatomedin Receptors
IGF Type 1 Receptor
In Situ Hybridization
Insulin Receptor
Insulin
Gene Expression
Olfactory Bulb
Brain
Messenger RNA
Ribonucleotides
Cerebellar Cortex
Pyramidal Cells
Dentate Gyrus
Receptor Protein-Tyrosine Kinases
Sensory Receptor Cells
Insulin-Like Growth Factor I
Protein-Tyrosine Kinases
Cerebellum
Hippocampus

Cite this

Mapping of brain insulin and insulin-like growth factor receptor gene expression by in situ hybridization. / Bondy, Carolyn A.; Bach, Mark A.; Lee, Wei Hua.

In: Neuroprotocols, Vol. 1, No. 3, 1992, p. 240-249.

Research output: Contribution to journalArticle

Bondy, Carolyn A. ; Bach, Mark A. ; Lee, Wei Hua. / Mapping of brain insulin and insulin-like growth factor receptor gene expression by in situ hybridization. In: Neuroprotocols. 1992 ; Vol. 1, No. 3. pp. 240-249.
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