Mapping of QTL influencing saccharin consumption in the selectively bred alcohol-preferring and -nonpreferring rat lines

Tatiana Foroud, P. Bice, P. Castelluccio, R. Bo, A. Ritchotte, R. Stewart, L. Lumeng, T. K. Li, L. Carr

Research output: Contribution to journalArticle

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Abstract

The inbred preferring (iP) and nonpreferring (iNP) rat strains were derived from the selectively bred alcohol-preferring (P) and alcohol-nonpreferring (NP) lines. Previously, 381 iP × iNP F2 progeny were generated to identify quantitative trait loci (QTLs) influencing alcohol consumption and preference. Saccharin consumption (ml/48 h) and saccharin intake (ml/kg/day) were also measured in the F2 sample and were significantly correlated with both alcohol consumption and preference (all r ≥ .20, p < .0001), suggesting that there might be some QTLs influencing both saccharin and alcohol phenotypes. We have performed a genome screen using F2 animals with extreme saccharin or alcohol consumption to identify QTLs contributing to saccharin-related phenotypes. Lod scores greater than 2.0 were found on chromosomes 3, 16 and 18 in this sample. Additional genotyping was performed in these regions in the full sample of 381 F2 progeny to further characterize these putative QTLs. On chromosome 3, the maximum lod score in the full sample was 2.7 with saccharin consumption. This QTL appears to overlap with a QTL identified for alcohol consumption in the iP and iNP lines and has pleiotropic effects on both phenotypes. Interestingly, this region of rat chromosome 3 is syntenic with mouse chromosome 2, where a QTL influencing alcohol preference has been previously reported. The QTL on chromosome 16 has a maximum lod score of 4.0 with saccharin intake and 2.6 with saccharin consumption. The QTL on chromosome 18 has a maximum lod score of 2.7 with saccharin consumption. Taken together, these data provide the first results of a genome screen for QTLs contributing to saccharin phenotypes in the rat.

Original languageEnglish
Pages (from-to)57-67
Number of pages11
JournalBehavior Genetics
Volume32
Issue number1
DOIs
StatePublished - 2002

Fingerprint

saccharin
Saccharin
Quantitative Trait Loci
alcohol
quantitative trait loci
alcohols
Alcohols
breeds
chromosome
rats
Lod Score
phenotype
chromosomes
Alcohol Drinking
Chromosomes, Human, Pair 3
Phenotype
Chromosomes, Human, Pair 18
Chromosomes, Human, Pair 16
genome
consumption

Keywords

  • Quantitative trait locus (QTL)
  • Saccharin

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)
  • Behavioral Neuroscience
  • Psychology(all)

Cite this

Mapping of QTL influencing saccharin consumption in the selectively bred alcohol-preferring and -nonpreferring rat lines. / Foroud, Tatiana; Bice, P.; Castelluccio, P.; Bo, R.; Ritchotte, A.; Stewart, R.; Lumeng, L.; Li, T. K.; Carr, L.

In: Behavior Genetics, Vol. 32, No. 1, 2002, p. 57-67.

Research output: Contribution to journalArticle

Foroud, T, Bice, P, Castelluccio, P, Bo, R, Ritchotte, A, Stewart, R, Lumeng, L, Li, TK & Carr, L 2002, 'Mapping of QTL influencing saccharin consumption in the selectively bred alcohol-preferring and -nonpreferring rat lines', Behavior Genetics, vol. 32, no. 1, pp. 57-67. https://doi.org/10.1023/A:1014459912935
Foroud, Tatiana ; Bice, P. ; Castelluccio, P. ; Bo, R. ; Ritchotte, A. ; Stewart, R. ; Lumeng, L. ; Li, T. K. ; Carr, L. / Mapping of QTL influencing saccharin consumption in the selectively bred alcohol-preferring and -nonpreferring rat lines. In: Behavior Genetics. 2002 ; Vol. 32, No. 1. pp. 57-67.
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