Marking IL-4-producing cells by knock-in of the IL-4 gene

I. Cheng Ho, Mark H. Kaplan, Laurie Jackson-Grusby, Laurie H. Glimcher, Michael J. Grusby

Research output: Contribution to journalArticle

7 Scopus citations


IL-4 is a cytokine which can be expressed by a number of cell types including T(h)2 cells, mast cells and a population of CD4+ NK1.1+ NK T cells. Although phenotypic markers exist for identifying each of these cell types, there is at present no known cell surface marker common to all IL-4-producing cells. Using gene targeting in embryonic stem cells, we have modified the IL-4 locus by knock-in of a transmembrane domain to generate mice that express a membrane-bound form of IL-4 (mIL-4). Flow cytometry using an IL-4-specific mAb allowed the detection of IL-4-secreting T(h)2 cells, mast cells and NK T cells from mIL-4 mice. Furthermore, the analysis of immune responses in mIL-4 mice following immunization with anti-CD3 and anti-lgD has allowed us to identify distinct subpopulations of IL-4-producing NK T cells. Thus, the expression of IL-4 in a membrane-bound form provides a novel method for the identification and characterization of IL-4-producing cells.

Original languageEnglish (US)
Pages (from-to)243-247
Number of pages5
JournalInternational immunology
Issue number2
StatePublished - 1999


  • Cytokines
  • T(h)1/T(h)2
  • Transgenic/knockout

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Ho, I. C., Kaplan, M. H., Jackson-Grusby, L., Glimcher, L. H., & Grusby, M. J. (1999). Marking IL-4-producing cells by knock-in of the IL-4 gene. International immunology, 11(2), 243-247.