Marking IL-4-producing cells by knock-in of the IL-4 gene

I. Cheng Ho, Mark Kaplan, Laurie Jackson-Grusby, Laurie H. Glimcher, Michael J. Grusby

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

IL-4 is a cytokine which can be expressed by a number of cell types including T(h)2 cells, mast cells and a population of CD4+ NK1.1+ NK T cells. Although phenotypic markers exist for identifying each of these cell types, there is at present no known cell surface marker common to all IL-4-producing cells. Using gene targeting in embryonic stem cells, we have modified the IL-4 locus by knock-in of a transmembrane domain to generate mice that express a membrane-bound form of IL-4 (mIL-4). Flow cytometry using an IL-4-specific mAb allowed the detection of IL-4-secreting T(h)2 cells, mast cells and NK T cells from mIL-4 mice. Furthermore, the analysis of immune responses in mIL-4 mice following immunization with anti-CD3 and anti-lgD has allowed us to identify distinct subpopulations of IL-4-producing NK T cells. Thus, the expression of IL-4 in a membrane-bound form provides a novel method for the identification and characterization of IL-4-producing cells.

Original languageEnglish (US)
Pages (from-to)243-247
Number of pages5
JournalInternational Immunology
Volume11
Issue number2
DOIs
StatePublished - 1999
Externally publishedYes

Fingerprint

Interleukin-4
Genes
Natural Killer Cells
T-Lymphocytes
Mast Cells
Membranes
Gene Targeting
Embryonic Stem Cells
Immunization
Flow Cytometry
Cell Count
Cell Membrane
Cytokines

Keywords

  • Cytokines
  • T(h)1/T(h)2
  • Transgenic/knockout

ASJC Scopus subject areas

  • Immunology

Cite this

Ho, I. C., Kaplan, M., Jackson-Grusby, L., Glimcher, L. H., & Grusby, M. J. (1999). Marking IL-4-producing cells by knock-in of the IL-4 gene. International Immunology, 11(2), 243-247. https://doi.org/10.1093/intimm/11.2.243

Marking IL-4-producing cells by knock-in of the IL-4 gene. / Ho, I. Cheng; Kaplan, Mark; Jackson-Grusby, Laurie; Glimcher, Laurie H.; Grusby, Michael J.

In: International Immunology, Vol. 11, No. 2, 1999, p. 243-247.

Research output: Contribution to journalArticle

Ho, IC, Kaplan, M, Jackson-Grusby, L, Glimcher, LH & Grusby, MJ 1999, 'Marking IL-4-producing cells by knock-in of the IL-4 gene', International Immunology, vol. 11, no. 2, pp. 243-247. https://doi.org/10.1093/intimm/11.2.243
Ho, I. Cheng ; Kaplan, Mark ; Jackson-Grusby, Laurie ; Glimcher, Laurie H. ; Grusby, Michael J. / Marking IL-4-producing cells by knock-in of the IL-4 gene. In: International Immunology. 1999 ; Vol. 11, No. 2. pp. 243-247.
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