Marrow-derived cells populate scaffolds composed of xenogeneic extracellular matrix

Stephen F. Badylak, Kinam Park, Nicholas Peppas, George McCabe, Mervin Yoder

Research output: Contribution to journalArticle

145 Citations (Scopus)

Abstract

Introduction. The source of cells that participate in wound repair directly affects outcome. The extracellular matrix (ECM) and other acellular biomaterials have been used as therapeutic scaffolds for cell attachment and proliferation and as templates for tissue repair. The ECM consists of structural and functional proteins that influence cell attachment, gene expression patterns, and the differentiation of cells. Objective. The objective of this study was to determine if the composition of acellular matrix scaffolds affects the recruitment of bone marrow-derived cellular elements that populate the scaffolds in vivo. Methods. Scaffolds composed of porcine tissue ECM, purified Type I collagen, poly(L)lactic coglycolic acid (PLGA), or a mixture of porcine ECM and PLGA were implanted into subcutaneous pouches on the dorsum of mice. The origin of cells that populated the matrices was determined by first performing bone marrow transplantation to convert the marrow of glucose phosphate isomerase 1b (Gpi-1 b) mice to cells expressing glucose phosphate isomerase 1a (Gpi-1 a). Results. A significant increase in Gpi-1 a expressing cells was present in sites implanted with the porcine ECM compared to sites implanted with either Type I collagen or PLGA. Use of recipient mice transplanted with marrow cells that expressed β-galactosidase confirmed that the majority of cells that populated and remodeled the naturally occurring porcine ECM were marrow derived. Addition of porcine ECM to the PLGA scaffold caused a significant increase in the number of marrow-derived cells that became part of the remodeled implant site. Conclusion. The composition of bioscaffolds affects the cellular recruitment pattern during tissue repair. ECM scaffolds facilitate the recruitment of marrow-derived cells into sites of remodeling.

Original languageEnglish
Pages (from-to)1310-1318
Number of pages9
JournalExperimental Hematology
Volume29
Issue number11
DOIs
StatePublished - 2001

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Extracellular Matrix
Bone Marrow
xylose isomerase
Swine
Phosphates
Collagen Type I
Galactosidases
Biocompatible Materials
Bone Marrow Transplantation
Cell Differentiation
Cell Proliferation
Gene Expression
polylactic acid-polyglycolic acid copolymer
Wounds and Injuries
Proteins

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Genetics
  • Hematology
  • Oncology
  • Transplantation

Cite this

Marrow-derived cells populate scaffolds composed of xenogeneic extracellular matrix. / Badylak, Stephen F.; Park, Kinam; Peppas, Nicholas; McCabe, George; Yoder, Mervin.

In: Experimental Hematology, Vol. 29, No. 11, 2001, p. 1310-1318.

Research output: Contribution to journalArticle

Badylak, Stephen F. ; Park, Kinam ; Peppas, Nicholas ; McCabe, George ; Yoder, Mervin. / Marrow-derived cells populate scaffolds composed of xenogeneic extracellular matrix. In: Experimental Hematology. 2001 ; Vol. 29, No. 11. pp. 1310-1318.
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