Mass spectrometry for protein quantification in biomarker discovery

Mu Wang, Jinsam You

Research output: Chapter in Book/Report/Conference proceedingChapter

14 Scopus citations

Abstract

Major technological advances have made proteomics an extremely active field for biomarker discovery in recent years due primarily to the development of newer mass spectrometric technologies and the explosion in genomic and protein bioinformatics. This leads to an increased emphasis on larger scale, faster, and more efficient methods for detecting protein biomarkers in human tissues, cells, and biofluids. Most current proteomic methodologies for biomarker discovery, however, are not highly automated and are generally labor-intensive and expensive. More automation and improved software programs capable of handling a large amount of data are essential to reduce the cost of discovery and to increase throughput. In this chapter, we discuss and describe mass spectrometry-based proteomic methods for quantitative protein analysis.

Original languageEnglish (US)
Title of host publicationFunctional Genomics
Subtitle of host publicationMethods and Protocols
EditorsMichael Kaufmann, Claudia Klinger
Pages199-225
Number of pages27
DOIs
StatePublished - Jan 2 2012

Publication series

NameMethods in Molecular Biology
Volume815
ISSN (Print)1064-3745

Keywords

  • Biomarkers
  • Label-free protein quantification
  • Mass spectrometry
  • Proteomics
  • Stable isotope labeling

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Wang, M., & You, J. (2012). Mass spectrometry for protein quantification in biomarker discovery. In M. Kaufmann, & C. Klinger (Eds.), Functional Genomics: Methods and Protocols (pp. 199-225). (Methods in Molecular Biology; Vol. 815). https://doi.org/10.1007/978-1-61779-424-7_16