Mass spectrometry for protein quantification in biomarker discovery

Mu Wang, Jinsam You

Research output: Chapter in Book/Report/Conference proceedingChapter

14 Citations (Scopus)

Abstract

Major technological advances have made proteomics an extremely active field for biomarker discovery in recent years due primarily to the development of newer mass spectrometric technologies and the explosion in genomic and protein bioinformatics. This leads to an increased emphasis on larger scale, faster, and more efficient methods for detecting protein biomarkers in human tissues, cells, and biofluids. Most current proteomic methodologies for biomarker discovery, however, are not highly automated and are generally labor-intensive and expensive. More automation and improved software programs capable of handling a large amount of data are essential to reduce the cost of discovery and to increase throughput. In this chapter, we discuss and describe mass spectrometry-based proteomic methods for quantitative protein analysis.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Pages199-225
Number of pages27
Volume815
DOIs
StatePublished - 2012

Publication series

NameMethods in Molecular Biology
Volume815
ISSN (Print)10643745

Fingerprint

Proteomics
Mass Spectrometry
Biomarkers
Proteins
Explosions
Automation
Computational Biology
Software
Technology
Costs and Cost Analysis

Keywords

  • Biomarkers
  • Label-free protein quantification
  • Mass spectrometry
  • Proteomics
  • Stable isotope labeling

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Wang, M., & You, J. (2012). Mass spectrometry for protein quantification in biomarker discovery. In Methods in Molecular Biology (Vol. 815, pp. 199-225). (Methods in Molecular Biology; Vol. 815). https://doi.org/10.1007/978-1-61779-424-7_16

Mass spectrometry for protein quantification in biomarker discovery. / Wang, Mu; You, Jinsam.

Methods in Molecular Biology. Vol. 815 2012. p. 199-225 (Methods in Molecular Biology; Vol. 815).

Research output: Chapter in Book/Report/Conference proceedingChapter

Wang, M & You, J 2012, Mass spectrometry for protein quantification in biomarker discovery. in Methods in Molecular Biology. vol. 815, Methods in Molecular Biology, vol. 815, pp. 199-225. https://doi.org/10.1007/978-1-61779-424-7_16
Wang M, You J. Mass spectrometry for protein quantification in biomarker discovery. In Methods in Molecular Biology. Vol. 815. 2012. p. 199-225. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-61779-424-7_16
Wang, Mu ; You, Jinsam. / Mass spectrometry for protein quantification in biomarker discovery. Methods in Molecular Biology. Vol. 815 2012. pp. 199-225 (Methods in Molecular Biology).
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