Mast cells mediate the severity of experimental cystitis in mice

D. E. Bjorling, Travis Jerde, M. J. Zine, B. W. Busser, M. R. Saban, R. Saban

Research output: Contribution to journalArticle

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Abstract

Purpose: We hypothesized that experimental cystitis induced by substance P (SP) or E. coli lipopolysaccharide (LPS) would be less severe in mice rendered mast cell deficient by genetic manipulation. Materials and Methods: Two strains of mast-cell deficient mice (WBB6F1- kit(W)/kit(W-v) or kit(W)/kit(W-v) and WCB6F1-S1/Sld or Sl/Sld) and their congenic, normal (+/+) counterparts were used. Cystitis was induced in female mice by intravenous injection of SP (0.1 ml.; 10-6 M) or E. coli LPS (0.1 ml.; 2 mg./ml.), and inflammation was assessed by Evans blue dye extravasation. In a separate group of kit(W)/kit(W-v) and congenic normal mice, cystitis was induced by intravesical infusion of SP (0.05 ml.; 10-5 M) or E. coli LPS (0.05 ml.; 100 μg./ml.) and compared with intravesical pyrogen-free saline (0.05 ml.; 0.9%). Severity of cystitis was determined by histological evaluation of the bladder wall 24 hours after intravesical infusions. Results: Intravenous SP or LPS stimulated increased plasma extravasation in congenic normal mice but not in mast cell-deficient mice. Intravesical SP or LPS resulted in increased edema, leukocytic infiltration, and hemorrhage within the bladder wall in congenic normal mice, but the only histological evidence of inflammation in the bladders of kit(W)/kit(W-v) mice was increased hemorrhage in response to LPS. Conclusions: This study indicates that mast cells modulate the inflammatory response of the bladder to SP and LPS in mice. Although clinical trials of the use of antihistamines to treat or prevent cystitis have not been successful, these results suggest that therapies directed toward preventing mast cell activation may yet prove effective in treating cystitis.

Original languageEnglish (US)
Pages (from-to)231-236
Number of pages6
JournalJournal of Urology
Volume162
Issue number1
DOIs
StatePublished - 1999
Externally publishedYes

Fingerprint

Cystitis
Mast Cells
Substance P
Lipopolysaccharides
Congenic Mice
Urinary Bladder
Escherichia coli
Hemorrhage
Inflammation
Pyrogens
Evans Blue
Histamine Antagonists
Intravenous Injections
Edema
Coloring Agents
Clinical Trials

Keywords

  • Cystitis
  • Lipopolysaccharide
  • Mast cells
  • Substance P

ASJC Scopus subject areas

  • Urology

Cite this

Bjorling, D. E., Jerde, T., Zine, M. J., Busser, B. W., Saban, M. R., & Saban, R. (1999). Mast cells mediate the severity of experimental cystitis in mice. Journal of Urology, 162(1), 231-236. https://doi.org/10.1097/00005392-199907000-00073

Mast cells mediate the severity of experimental cystitis in mice. / Bjorling, D. E.; Jerde, Travis; Zine, M. J.; Busser, B. W.; Saban, M. R.; Saban, R.

In: Journal of Urology, Vol. 162, No. 1, 1999, p. 231-236.

Research output: Contribution to journalArticle

Bjorling, DE, Jerde, T, Zine, MJ, Busser, BW, Saban, MR & Saban, R 1999, 'Mast cells mediate the severity of experimental cystitis in mice', Journal of Urology, vol. 162, no. 1, pp. 231-236. https://doi.org/10.1097/00005392-199907000-00073
Bjorling, D. E. ; Jerde, Travis ; Zine, M. J. ; Busser, B. W. ; Saban, M. R. ; Saban, R. / Mast cells mediate the severity of experimental cystitis in mice. In: Journal of Urology. 1999 ; Vol. 162, No. 1. pp. 231-236.
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abstract = "Purpose: We hypothesized that experimental cystitis induced by substance P (SP) or E. coli lipopolysaccharide (LPS) would be less severe in mice rendered mast cell deficient by genetic manipulation. Materials and Methods: Two strains of mast-cell deficient mice (WBB6F1- kit(W)/kit(W-v) or kit(W)/kit(W-v) and WCB6F1-S1/Sld or Sl/Sld) and their congenic, normal (+/+) counterparts were used. Cystitis was induced in female mice by intravenous injection of SP (0.1 ml.; 10-6 M) or E. coli LPS (0.1 ml.; 2 mg./ml.), and inflammation was assessed by Evans blue dye extravasation. In a separate group of kit(W)/kit(W-v) and congenic normal mice, cystitis was induced by intravesical infusion of SP (0.05 ml.; 10-5 M) or E. coli LPS (0.05 ml.; 100 μg./ml.) and compared with intravesical pyrogen-free saline (0.05 ml.; 0.9{\%}). Severity of cystitis was determined by histological evaluation of the bladder wall 24 hours after intravesical infusions. Results: Intravenous SP or LPS stimulated increased plasma extravasation in congenic normal mice but not in mast cell-deficient mice. Intravesical SP or LPS resulted in increased edema, leukocytic infiltration, and hemorrhage within the bladder wall in congenic normal mice, but the only histological evidence of inflammation in the bladders of kit(W)/kit(W-v) mice was increased hemorrhage in response to LPS. Conclusions: This study indicates that mast cells modulate the inflammatory response of the bladder to SP and LPS in mice. Although clinical trials of the use of antihistamines to treat or prevent cystitis have not been successful, these results suggest that therapies directed toward preventing mast cell activation may yet prove effective in treating cystitis.",
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AU - Saban, M. R.

AU - Saban, R.

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