Mast Cells Regulate Epidermal Barrier Function and the Development of Allergic Skin Inflammation

Sarita Sehra, Ana P M Serezani, Jesus A. Ocaña, Jeffrey Travers, Mark Kaplan

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Atopic dermatitis is a chronic inflammatory skin disease characterized by infiltration of eosinophils, T helper cells, and mast cells. The role of mast cells in atopic dermatitis is not completely understood. To define the effects of mast cells on skin biology, we observed that mast cells regulate the homeostatic expression of epidermal differentiation complex and other skin genes. Decreased epidermal differentiation complex gene expression in mice that genetically lack mast cells (KitW–sh/W–sh mice) is associated with increased uptake of protein antigens painted on the skin by dendritic cells (DCs) compared with similarly treated wild-type mice, suggesting a protective role for mast cells in exposure to nominal environmental allergens. To test this further, we crossed KitW–sh/W–sh mice with signal transducer and activator of transcription 6 (i.e., Stat6) VT transgenic mice that develop spontaneous atopic dermatitis-like disease that is dependent on T helper cell 2 cytokines and is associated with high serum concentrations of IgE. We observed that Stat6VT × KitW–sh/W–sh mice developed more frequent and more severe allergic skin inflammation than Stat6VT transgenic mice that had mast cells. Together, these studies suggest that mast cells regulate epidermal barrier function and have a potential protective role in the development of atopic dermatitis-like disease.

Original languageEnglish (US)
Pages (from-to)1429-1437
Number of pages9
JournalJournal of Investigative Dermatology
Volume136
Issue number7
DOIs
StatePublished - Jul 1 2016

Fingerprint

Mast Cells
Skin
Inflammation
Atopic Dermatitis
STAT6 Transcription Factor
Transgenic Mice
Infiltration
Gene expression
Allergens
Immunoglobulin E
Th2 Cells
Genes
Langerhans Cells
Cytokines
Antigens
Helper-Inducer T-Lymphocytes
Skin Diseases
Eosinophils
Gene Expression
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology
  • Cell Biology

Cite this

Mast Cells Regulate Epidermal Barrier Function and the Development of Allergic Skin Inflammation. / Sehra, Sarita; Serezani, Ana P M; Ocaña, Jesus A.; Travers, Jeffrey; Kaplan, Mark.

In: Journal of Investigative Dermatology, Vol. 136, No. 7, 01.07.2016, p. 1429-1437.

Research output: Contribution to journalArticle

@article{d876685859c640e3a11d72d1ebd536bd,
title = "Mast Cells Regulate Epidermal Barrier Function and the Development of Allergic Skin Inflammation",
abstract = "Atopic dermatitis is a chronic inflammatory skin disease characterized by infiltration of eosinophils, T helper cells, and mast cells. The role of mast cells in atopic dermatitis is not completely understood. To define the effects of mast cells on skin biology, we observed that mast cells regulate the homeostatic expression of epidermal differentiation complex and other skin genes. Decreased epidermal differentiation complex gene expression in mice that genetically lack mast cells (KitW–sh/W–sh mice) is associated with increased uptake of protein antigens painted on the skin by dendritic cells (DCs) compared with similarly treated wild-type mice, suggesting a protective role for mast cells in exposure to nominal environmental allergens. To test this further, we crossed KitW–sh/W–sh mice with signal transducer and activator of transcription 6 (i.e., Stat6) VT transgenic mice that develop spontaneous atopic dermatitis-like disease that is dependent on T helper cell 2 cytokines and is associated with high serum concentrations of IgE. We observed that Stat6VT × KitW–sh/W–sh mice developed more frequent and more severe allergic skin inflammation than Stat6VT transgenic mice that had mast cells. Together, these studies suggest that mast cells regulate epidermal barrier function and have a potential protective role in the development of atopic dermatitis-like disease.",
author = "Sarita Sehra and Serezani, {Ana P M} and Oca{\~n}a, {Jesus A.} and Jeffrey Travers and Mark Kaplan",
year = "2016",
month = "7",
day = "1",
doi = "10.1016/j.jid.2016.03.019",
language = "English (US)",
volume = "136",
pages = "1429--1437",
journal = "Journal of Investigative Dermatology",
issn = "0022-202X",
publisher = "Nature Publishing Group",
number = "7",

}

TY - JOUR

T1 - Mast Cells Regulate Epidermal Barrier Function and the Development of Allergic Skin Inflammation

AU - Sehra, Sarita

AU - Serezani, Ana P M

AU - Ocaña, Jesus A.

AU - Travers, Jeffrey

AU - Kaplan, Mark

PY - 2016/7/1

Y1 - 2016/7/1

N2 - Atopic dermatitis is a chronic inflammatory skin disease characterized by infiltration of eosinophils, T helper cells, and mast cells. The role of mast cells in atopic dermatitis is not completely understood. To define the effects of mast cells on skin biology, we observed that mast cells regulate the homeostatic expression of epidermal differentiation complex and other skin genes. Decreased epidermal differentiation complex gene expression in mice that genetically lack mast cells (KitW–sh/W–sh mice) is associated with increased uptake of protein antigens painted on the skin by dendritic cells (DCs) compared with similarly treated wild-type mice, suggesting a protective role for mast cells in exposure to nominal environmental allergens. To test this further, we crossed KitW–sh/W–sh mice with signal transducer and activator of transcription 6 (i.e., Stat6) VT transgenic mice that develop spontaneous atopic dermatitis-like disease that is dependent on T helper cell 2 cytokines and is associated with high serum concentrations of IgE. We observed that Stat6VT × KitW–sh/W–sh mice developed more frequent and more severe allergic skin inflammation than Stat6VT transgenic mice that had mast cells. Together, these studies suggest that mast cells regulate epidermal barrier function and have a potential protective role in the development of atopic dermatitis-like disease.

AB - Atopic dermatitis is a chronic inflammatory skin disease characterized by infiltration of eosinophils, T helper cells, and mast cells. The role of mast cells in atopic dermatitis is not completely understood. To define the effects of mast cells on skin biology, we observed that mast cells regulate the homeostatic expression of epidermal differentiation complex and other skin genes. Decreased epidermal differentiation complex gene expression in mice that genetically lack mast cells (KitW–sh/W–sh mice) is associated with increased uptake of protein antigens painted on the skin by dendritic cells (DCs) compared with similarly treated wild-type mice, suggesting a protective role for mast cells in exposure to nominal environmental allergens. To test this further, we crossed KitW–sh/W–sh mice with signal transducer and activator of transcription 6 (i.e., Stat6) VT transgenic mice that develop spontaneous atopic dermatitis-like disease that is dependent on T helper cell 2 cytokines and is associated with high serum concentrations of IgE. We observed that Stat6VT × KitW–sh/W–sh mice developed more frequent and more severe allergic skin inflammation than Stat6VT transgenic mice that had mast cells. Together, these studies suggest that mast cells regulate epidermal barrier function and have a potential protective role in the development of atopic dermatitis-like disease.

UR - http://www.scopus.com/inward/record.url?scp=84980354548&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84980354548&partnerID=8YFLogxK

U2 - 10.1016/j.jid.2016.03.019

DO - 10.1016/j.jid.2016.03.019

M3 - Article

C2 - 27021404

AN - SCOPUS:84980354548

VL - 136

SP - 1429

EP - 1437

JO - Journal of Investigative Dermatology

JF - Journal of Investigative Dermatology

SN - 0022-202X

IS - 7

ER -