Matrix metalloproteinase expression during blastema formation in regeneration-competent versus regeneration-deficient amphibian limbs

Neetha Santosh, L. Windsor, Behnaz S. Mahmoudi, Bingbing Li, Weiping Zhang, Ellen A. Chernoff, Nandini Rao, David L. Stocum, Fengyu Song

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

We used an antibody array to compare the protein expression of matrix metalloproteinases (MMPs)-1, -2, -3, -8, -9, -10, and -13, as well as the tissue inhibitors of metalloproteinases (TIMPs)-1, -2, and -4 during blastema formation in amputated hindlimbs of regeneration-competent wild-type axolotls and stage-54 Xenopus, and regeneration-deficient short-toes axolotls and Xenopus froglets. Expression of MMP-9 and -2 was also compared by zymography. Both short-toes and froglet failed to up-regulate MMPs in a pattern comparable to the wild-type axolotl, suggesting that subnormal histolysis is at least in part responsible for the poor blastema formation characteristic of both short-toes and froglet. MMP levels were much lower in amputated stage-54 Xenopus limb buds than in the other animals, suggesting that blastema formation in these limb buds requires much less extracellular matrix degradation than in fully differentiated limbs. TIMP expression patterns followed the same trends as the MMP's in each group of animals.

Original languageEnglish (US)
Pages (from-to)1127-1141
Number of pages15
JournalDevelopmental Dynamics
Volume240
Issue number5
DOIs
StatePublished - May 2011

Fingerprint

Ambystoma mexicanum
Toes
Amphibians
Xenopus
Matrix Metalloproteinases
Limb Buds
Regeneration
Extremities
Matrix Metalloproteinase 2
Tissue Inhibitor of Metalloproteinases
Tissue Inhibitor of Metalloproteinase-2
Matrix Metalloproteinase 1
Tissue Inhibitor of Metalloproteinase-1
Matrix Metalloproteinase 9
Hindlimb
Extracellular Matrix
Up-Regulation
Antibodies
Proteins

Keywords

  • Amphibian
  • Blastema
  • Histolysis
  • Limb regeneration
  • Matrix metalloproteinases

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Matrix metalloproteinase expression during blastema formation in regeneration-competent versus regeneration-deficient amphibian limbs. / Santosh, Neetha; Windsor, L.; Mahmoudi, Behnaz S.; Li, Bingbing; Zhang, Weiping; Chernoff, Ellen A.; Rao, Nandini; Stocum, David L.; Song, Fengyu.

In: Developmental Dynamics, Vol. 240, No. 5, 05.2011, p. 1127-1141.

Research output: Contribution to journalArticle

Santosh, Neetha ; Windsor, L. ; Mahmoudi, Behnaz S. ; Li, Bingbing ; Zhang, Weiping ; Chernoff, Ellen A. ; Rao, Nandini ; Stocum, David L. ; Song, Fengyu. / Matrix metalloproteinase expression during blastema formation in regeneration-competent versus regeneration-deficient amphibian limbs. In: Developmental Dynamics. 2011 ; Vol. 240, No. 5. pp. 1127-1141.
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