Matrix metalloproteinases

Henning Birkedal-Hansen, Susan Yamada, L. Windsor, Anne Havernose Pollard, Guy Lyons, William Stetler-Stevenson, Bente Birkedal-Hansen

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Matrix metalloproteinases are a class of enzymes that play an important role in the remodeling of the extracellular matrix in development and cancer metastasis. This unit describes a set of methods-cell-mediated dissolution of type-1 collagen fibrils, direct and reverse zymography, enzyme capture based on α 2-macroglobulin and TIMP-1 and -2, and demonstration of cryptic thiol groups in metalloproteinase precursors-that are used to characterize the functions of matrix metalloproteinases and their inhibitors.

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Matrix Metalloproteinases
Tissue Inhibitor of Metalloproteinase-2
Macroglobulins
Tissue Inhibitor of Metalloproteinase-1
Matrix Metalloproteinase Inhibitors
Metalloproteases
Enzymes
Collagen Type I
Sulfhydryl Compounds
Extracellular Matrix
Neoplasm Metastasis
Neoplasms

Keywords

  • α2- macroglobulin
  • Matrix metalloproteinases
  • TIMP-1 and -2
  • Type-1 collagen
  • Zymography

ASJC Scopus subject areas

  • Cell Biology
  • Medicine(all)

Cite this

Birkedal-Hansen, H., Yamada, S., Windsor, L., Pollard, A. H., Lyons, G., Stetler-Stevenson, W., & Birkedal-Hansen, B. (2008). Matrix metalloproteinases. Current protocols in cell biology / editorial board, Juan S. Bonifacino ... [et al.], (SUPPL. 40). https://doi.org/10.1002/0471143030.cb1008s40

Matrix metalloproteinases. / Birkedal-Hansen, Henning; Yamada, Susan; Windsor, L.; Pollard, Anne Havernose; Lyons, Guy; Stetler-Stevenson, William; Birkedal-Hansen, Bente.

In: Current protocols in cell biology / editorial board, Juan S. Bonifacino ... [et al.], No. SUPPL. 40, 2008.

Research output: Contribution to journalArticle

Birkedal-Hansen, H, Yamada, S, Windsor, L, Pollard, AH, Lyons, G, Stetler-Stevenson, W & Birkedal-Hansen, B 2008, 'Matrix metalloproteinases', Current protocols in cell biology / editorial board, Juan S. Bonifacino ... [et al.], no. SUPPL. 40. https://doi.org/10.1002/0471143030.cb1008s40
Birkedal-Hansen, Henning ; Yamada, Susan ; Windsor, L. ; Pollard, Anne Havernose ; Lyons, Guy ; Stetler-Stevenson, William ; Birkedal-Hansen, Bente. / Matrix metalloproteinases. In: Current protocols in cell biology / editorial board, Juan S. Bonifacino ... [et al.]. 2008 ; No. SUPPL. 40.
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