Matrix metalloproteinases

Henning Birkedal-Hansen, Susan Yamada, Jack Windsor, Anne Havernose Pollard, Guy Lyons, William Stetler-Stevenson, Bente Birkedal-Hansen

Research output: Contribution to journalReview article

33 Scopus citations


Matrix metalloproteinases are a class of enzymes that play an important role in the remodeling of the extracellular matrix in development and cancer metastasis. This unit describes a set of methods-cell-mediated dissolution of type-1 collagen fibrils, direct and reverse zymography, enzyme capture based on α 2-macroglobulin and TIMP-1 and -2, and demonstration of cryptic thiol groups in metalloproteinase precursors-that are used to characterize the functions of matrix metalloproteinases and their inhibitors.

Original languageEnglish (US)
Pages (from-to)10. 8.1-10. 8.23
JournalCurrent Protocols in Cell Biology
Issue numberSUPPL. 40
StatePublished - Jan 1 2008


  • Matrix metalloproteinases
  • TIMP-1 and -2
  • Type-1 collagen
  • Zymography
  • α2- macroglobulin

ASJC Scopus subject areas

  • Cell Biology

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  • Cite this

    Birkedal-Hansen, H., Yamada, S., Windsor, J., Pollard, A. H., Lyons, G., Stetler-Stevenson, W., & Birkedal-Hansen, B. (2008). Matrix metalloproteinases. Current Protocols in Cell Biology, (SUPPL. 40), 10. 8.1-10. 8.23.