Matrix rigidity regulates spatiotemporal dynamics of Cdc42 activity and vacuole formation kinetics of endothelial colony forming cells

Seung Joon Kim, Qiaoqiao Wan, Eunhye Cho, Bumsoo Han, Mervin Yoder, Sherry L. Voytik-Harbin, Sungsoo Na

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Recent evidence has shown that endothelial colony forming cells (ECFCs) may serve as a cell therapy for improving blood vessel formation in subjects with vascular injury, largely due to their robust vasculogenic potential. The Rho family GTPase Cdc42 is known to play a primary role in this vasculogenesis process, but little is known about how extracellular matrix (ECM) rigidity affects Cdc42 activity during the process. In this study, we addressed two questions: Does matrix rigidity affect Cdc42 activity in ECFC undergoing early vacuole formation? How is the spatiotemporal activation of Cdc42 related to ECFC vacuole formation? A fluorescence resonance energy transfer (FRET)-based Cdc42 biosensor was used to examine the effects of the rigidity of three-dimensional (3D) collagen matrices on spatiotemporal activity of Cdc42 in ECFCs. Collagen matrix stiffness was modulated by varying the collagen concentration and therefore fibril density. The results showed that soft (150 Pa) matrices induced an increased level of Cdc42 activity compared to stiff (1 kPa) matrices. Time-course imaging and colocalization analysis of Cdc42 activity and vacuole formation revealed that Cdc42 activity was colocalized to the periphery of cytoplasmic vacuoles. Moreover, soft matrices generated faster and larger vacuoles than stiff matrices. The matrix-driven vacuole formation was enhanced by a constitutively active Cdc42 mutant, but significantly inhibited by a dominant-negative Cdc42 mutant. Collectively, the results suggest that matrix rigidity is a strong regulator of Cdc42 activity and vacuole formation kinetics, and that enhanced activity of Cdc42 is an important step in early vacuole formation in ECFCs.

Original languageEnglish
Pages (from-to)1280-1285
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume443
Issue number4
DOIs
StatePublished - Jan 24 2014

Fingerprint

Vacuoles
Rigidity
Kinetics
Collagen
rho GTP-Binding Proteins
Fluorescence Resonance Energy Transfer
Vascular System Injuries
Biosensing Techniques
Cell- and Tissue-Based Therapy
GTP Phosphohydrolases
Blood vessels
Stiffness matrix
Extracellular Matrix
Blood Vessels
Biosensors
Chemical activation
Imaging techniques

Keywords

  • Endothelial colony forming cells (ECFCs)
  • Fluorescence resonance energy transfer (FRET)
  • Live cell imaging
  • Matrix stiffness
  • Mechanotransduction
  • Rho family GTPases

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Matrix rigidity regulates spatiotemporal dynamics of Cdc42 activity and vacuole formation kinetics of endothelial colony forming cells. / Kim, Seung Joon; Wan, Qiaoqiao; Cho, Eunhye; Han, Bumsoo; Yoder, Mervin; Voytik-Harbin, Sherry L.; Na, Sungsoo.

In: Biochemical and Biophysical Research Communications, Vol. 443, No. 4, 24.01.2014, p. 1280-1285.

Research output: Contribution to journalArticle

Kim, Seung Joon ; Wan, Qiaoqiao ; Cho, Eunhye ; Han, Bumsoo ; Yoder, Mervin ; Voytik-Harbin, Sherry L. ; Na, Sungsoo. / Matrix rigidity regulates spatiotemporal dynamics of Cdc42 activity and vacuole formation kinetics of endothelial colony forming cells. In: Biochemical and Biophysical Research Communications. 2014 ; Vol. 443, No. 4. pp. 1280-1285.
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AU - Yoder, Mervin

AU - Voytik-Harbin, Sherry L.

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