Maturation and localization of macrophages and microglia during infection with a neurotropic murine coronavirus

Steven P. Templeton, Taeg S. Kim, Katherine O'Malley, Stanley Perlman

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Macrophages and microglia are critical in the acute inflammatory response and act as final effector cells of demyelination during chronic infection with the neutrotropic MHV-JHM strain of mouse hepatitis virus (MHV-JHM). Herein, we show that "immature" F4/80+Ly-6Chi monocytes are the first cells, along with neutrophils, to enter the MHV-JHM-infected central nervous system (CNS). As the infection progresses, macrophages in the CNS down-regulate expression of Ly-6C and CD62L, consistent with maturation, and a higher frequency express CD11c, a marker for dendritic cells (DCs). Microglia also express CD11c during this phase of the infection. CD11c+ macrophages in the infected CNS exhibit variable properties of immature antigen-presenting cells (APCs), with modestly increased CD40 and MHC expression, and equivalent potent antigen uptake when compared with CD11c - macrophages. Furthermore, CDllc+ and F4/80+ macrophages and microglia are localized to areas of demyelination, in some instances directly associated with damaged axons. These results suggest that chronic CNS infection results in the appearance of CD11c-expressing macrophages from the blood that exhibit properties of immature APCs, are closely associated with areas of demyelination, and may act as final effectors of myelin destruction.

Original languageEnglish (US)
Pages (from-to)40-51
Number of pages12
JournalBrain Pathology
Volume18
Issue number1
DOIs
StatePublished - Jan 1 2008

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Coronavirus
Microglia
Murine hepatitis virus
Macrophages
Demyelinating Diseases
Infection
Central Nervous System
Antigen-Presenting Cells
Central Nervous System Infections
Myelin Sheath
Dendritic Cells
Axons
Monocytes
Neutrophils
Down-Regulation
Antigens

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Neuroscience(all)
  • Clinical Neurology

Cite this

Maturation and localization of macrophages and microglia during infection with a neurotropic murine coronavirus. / Templeton, Steven P.; Kim, Taeg S.; O'Malley, Katherine; Perlman, Stanley.

In: Brain Pathology, Vol. 18, No. 1, 01.01.2008, p. 40-51.

Research output: Contribution to journalArticle

Templeton, Steven P. ; Kim, Taeg S. ; O'Malley, Katherine ; Perlman, Stanley. / Maturation and localization of macrophages and microglia during infection with a neurotropic murine coronavirus. In: Brain Pathology. 2008 ; Vol. 18, No. 1. pp. 40-51.
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