Maturation of the proximal tubule in the puppy kidney: A comparison to the adult

D. A. Hay, Andrew Evan

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Two- to four-day-old beagle puppy kidneys were prepared for transmission and scanning electron microscopy and compared to similarly prepared adult tissues. Proximal tubules of puppy kidneys which contained nephrons in various stages of differentiation were examined and maturational changes were described. Lateral surface contours of proximal tubular cells were initially smooth and relatively unfolded, but progressively acquired complex processes that may be recognized as lateral ridges and lateral-basal processes. Basal projections began as short, stubby processes and gradually took on either a narrow, plate-like or finger-like appearance. Mitochondria, lysosomes and apical vacuoles increased in number as the tubules matured. Mitochondria lacked orientation in outer cortical tubules, but exhibited some vertical arrangement within basal processes in inner cortical tubules. Despite features indicating advanced maturation of tubules in the inner cortex, puppy kidneys lacked the lipid droplets characteristic of the adult. Thus, differentiation of this portion of the developing nephron into S1, S2, and S3 segments was not possible at day 4. Morphometric analyses of the lateral and basal membrane surface concentration of proximal convoluted tubules from the puppy revealed all cells to have a significantly smaller membrane area than that of the adult. However, the inner cortical cells of the puppy had a greater surface concentration than those of the outer cortex. The reduced transport capacity of the puppy proximal tubule may be related to the lack of segmentation and/or reduced lateral-basal surface area.

Original languageEnglish (US)
Pages (from-to)273-299
Number of pages27
JournalAnatomical Record
Volume195
Issue number2
StatePublished - 1979
Externally publishedYes

Fingerprint

proximal tubules
puppies
Nephrons
kidneys
Kidney
Mitochondria
Kidney Cortex
nephrons
Proximal Kidney Tubule
Scanning Transmission Electron Microscopy
Membranes
Vacuoles
Lysosomes
cortex
mitochondria
lysosomes
cells
Beagle
basal area
droplets

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Anatomy

Cite this

Maturation of the proximal tubule in the puppy kidney : A comparison to the adult. / Hay, D. A.; Evan, Andrew.

In: Anatomical Record, Vol. 195, No. 2, 1979, p. 273-299.

Research output: Contribution to journalArticle

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