MDS1, a dosage suppressor of an mck1 mutant, encodes a putative yeast homolog of glycogen synthase kinase

John W. Puziss, Thomas A. Hardy, Robert B. Johnson, Peter Roach, Philip Hieter

Research output: Contribution to journalArticle

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Abstract

The yeast gene MCK1 encodes a serine/threonine protein kinase that is thought to function in regulating kinetochore activity and entry into meiosis. Disruption of MCK1 confers a cold-sensitive phenotype, a temperature-sensitive phenotype, and sensitivity to the microtubule-destabilizing drug benomyl and leads to loss of chromosomes during growth on benomyl. A dosage suppression selection was used to identify genes that, when present at high copy number, could suppress the cold-sensitive phenotype of mck1:HIS3 mutant cells. Several unique classes of clones were identified, and one of these, designated MDS1, has been characterized in some detail. Nucleotide sequence data reveal that MDS1 encodes a serine/threonine protein kinase that is highly homologous to the shaggy/zw3 kinase in Drosophila melanogaster and its functional homolog, glycogen synthase kinase 3, in rats. The presence of MDS1 in high copy number rescues both the cold-sensitive and the temperature-sensitive phenotypes, but not the benomyl-sensitive phenotype, associated with the disruption of MCK1. Analysis of strains harboring an mds1 null mutation demonstrates that MDS1 is not essential during normal vegetative growth but appears to be required for meiosis. Finally, in vitro experiments indicate that the proteins encoded by both MCK1 and MDS1 possess protein kinase activity with substrate specificity similar to that of mammalian glycogen synthase kinase 3.

Original languageEnglish
Pages (from-to)831-839
Number of pages9
JournalMolecular and Cellular Biology
Volume14
Issue number1
StatePublished - 1994

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Glycogen Synthase Kinases
Benomyl
Yeasts
Phenotype
Glycogen Synthase Kinase 3
Protein-Serine-Threonine Kinases
Meiosis
Kinetochores
Growth
Substrate Specificity
Drosophila melanogaster
Microtubules
Protein Kinases
Genes
Phosphotransferases
Clone Cells
Chromosomes
Mutation
Temperature
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

Cite this

MDS1, a dosage suppressor of an mck1 mutant, encodes a putative yeast homolog of glycogen synthase kinase. / Puziss, John W.; Hardy, Thomas A.; Johnson, Robert B.; Roach, Peter; Hieter, Philip.

In: Molecular and Cellular Biology, Vol. 14, No. 1, 1994, p. 831-839.

Research output: Contribution to journalArticle

Puziss, John W. ; Hardy, Thomas A. ; Johnson, Robert B. ; Roach, Peter ; Hieter, Philip. / MDS1, a dosage suppressor of an mck1 mutant, encodes a putative yeast homolog of glycogen synthase kinase. In: Molecular and Cellular Biology. 1994 ; Vol. 14, No. 1. pp. 831-839.
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