Measurement of circulating leptin and soluble leptin receptors

Research output: Chapter in Book/Report/Conference proceedingChapter


Shortly following the discovery of leptin methods were developed to measure this novel adipokine in the circulation. The finding of multiple splice variants of the leptin receptor gene, including an mRNA for a soluble leptin receptor, suggested the presence of a circulating leptin binding protein. It was subsequently demonstrated that leptin associated with a soluble leptin receptor binding protein, although the mechanism through which the soluble leptin receptor reaches the circulation differs in rodents and humans. The quantitation of circulating leptin and soluble leptin receptor is now routinely performed in many laboratories. This chapter details the development of assays to measure leptin and soluble leptin receptor and describes some of the key findings about these circulating factors.

Original languageEnglish (US)
Title of host publicationLeptin: Regulation and Clinical Applications
PublisherSpringer International Publishing
Number of pages5
ISBN (Print)9783319099156, 9783319099149
StatePublished - Jan 1 2015


  • Binding proteins
  • Free leptin index
  • Leptin
  • Radioimmunoassay
  • Soluble leptin receptor

ASJC Scopus subject areas

  • Medicine(all)

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    Considine, R. (2015). Measurement of circulating leptin and soluble leptin receptors. In Leptin: Regulation and Clinical Applications (pp. 39-43). Springer International Publishing.