Measurement of dopamine release with continuous infusion of [11C]raclopride: Optimization and signal-to-noise considerations

Hiroshi Watabe, Christopher J. Endres, Alan Breier, Bernard Schmall, William C. Eckelman, Richard E. Carson

Research output: Contribution to journalArticle

90 Scopus citations

Abstract

PET studies with [11C]raclopride provide an indirect measure of changes in synaptic dopamine. Previously, we used the bolus-plus-infusion (B/I) method to assess dopamine response from the percentage change in binding potential (ΔBP) before and after administration of amphetamine. The goal of this work is to optimize the measurement of changes in neurotransmitter with the B/I method by choosing the optimal timing for pre- and poststimulus scanning. Methods: Two sources of variability in ΔBP were considered: within-subject and between-subject noise. A noise model based on a phantom study and human data was used to evaluate the within-subject noise. For between-subject noise, simulated time-activity curves were generated from measured [11C]raclopride input functions. Optimal timing to measure ΔBP was determined and applied to human data. Results: According to the simulation study, the optimal scan times for preand poststimulus scans were 39-50 and 58-100 min, respectively. The optimal timing resulted in a 28% noise reduction compared with the original timing. By applying the optimal timing to human studies, the statistical significance of the difference in ΔBP between patients with schizophrenia and healthy volunteers increased from P = 0.038 to 0.012. Conclusion: Careful assessment of the sources of noise in receptor imaging studies can increase the sensitivity of the B/I method for the detection of biologic signals.

Original languageEnglish (US)
Pages (from-to)522-530
Number of pages9
JournalJournal of Nuclear Medicine
Volume41
Issue number3
StatePublished - Mar 1 2000
Externally publishedYes

Keywords

  • Amphetamine
  • Bolus/infusion
  • Noise
  • Optimization
  • [C]raclopride

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

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