Measurement of endogenous lysophosphatidic acid by ESI-MS/MS in plasma samples requires pre-separation of lysophosphatidylcholine

Zhenwen Zhao, Yan Xu

Research output: Contribution to journalArticle

39 Scopus citations

Abstract

The levels of lysophosphatidic acid (LPA) or lysophosphatidylcholine (LPC) in plasma have been shown to be markers for several human diseases, including cancers. Here we show that the presence of LPC or other lysophospholipids (LPLs) in lipids extracted from biological samples affects accurate measurement of endogenous LPA in biological samples. We report for the first time the artificial conversion of LPC and lysophosphatidylserine (LPS) to LPA at the ion source of electrospray ionization tandem mass spectrometry (ESI-MS/MS). To avoid the interference of LPC with the quantification of LPA, a method based on high-performance liquid chromatography (HPLC) separation of LPA from LPC has been developed.

Original languageEnglish (US)
Pages (from-to)3739-3742
Number of pages4
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume877
Issue number29
DOIs
StatePublished - Nov 1 2009

Keywords

  • High-performance liquid chromatography electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS)
  • Lysophosphatidic acid (LPA)
  • Lysophosphatidylcholine (LPC)

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry
  • Cell Biology
  • Clinical Biochemistry

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