Measurement of the Activity of the Atg4 Cysteine Proteases

M. Li, Y. Fu, Z. Yang, Xiao-Ming Yin

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

While only one Atg4 is present in yeast, there are four Atg4 homologues in human and in mouse with different substrate specificities and catalytic efficiencies. The molecule Atg4 is a type of cysteine protease, and is known for its crucial roles in cleavage of the Atg8 family proteins before they can be conjugated to phospholipids, and also in cleavage of the conjugated Atg8 molecules from the membrane, a process known as deconjugation. Both processes are required for the maximal efficiency in autophagosome biogenesis. Atg4 could thus be a target for intervention of the autophagy process. It is thus important to measure Atg4 activity to determine and to modulate the autophagy function. Here, we review the catalytic functions and regulatory mechanisms of human Atg4 proteases and discuss the methodology for analyzing Atg4 activity in details.

Original languageEnglish (US)
JournalMethods in Enzymology
DOIs
StateAccepted/In press - 2016

Fingerprint

Cysteine Proteases
Autophagy
Molecules
Substrate Specificity
Yeast
Phospholipids
Peptide Hydrolases
Yeasts
Membranes
Substrates
Proteins
Autophagosomes

Keywords

  • Atg4
  • Atg8
  • Enzymatic kinetics
  • FRET
  • GABARAP
  • GATE-16
  • High-throughput screening
  • LC3

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Measurement of the Activity of the Atg4 Cysteine Proteases. / Li, M.; Fu, Y.; Yang, Z.; Yin, Xiao-Ming.

In: Methods in Enzymology, 2016.

Research output: Contribution to journalArticle

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