Measurements of low oxygen tension in vitro and response of macrophages to levels applicable to peri-and postoperative treatment of traumatic brain injury

Gabi N. Waite, Stephane Egot-Lemaire, Mark Bouwens, Henry O. Owegi, Pradeep Narotam, Lee R. Waite

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Established clinical guidelines for treatment of severe traumatic brain injury aim at maintaining intracranial and cerebral perfusion pressures. Recently, it has been shown that additional regulation of cerebral oxygen delivery helps to decrease patient mortality and leads to improved 6-month quality-of-life scores. However, eubaric oxygen-guided therapy is still controversial since it is well known that hyperoxia can cause unwanted secondary brain injury. Research studies are warranted to better understand the range of oxygen pressures that positively influence brain cell behavior. We perform such studies using a two-enzyme in vitro system that allows exposing tissue culture cells to various steady-state, or rapidly changing, oxygen pressures. Here, we present a mathematical model of the system and its validation by real-time monitoring of oxygen tensions. We additionally present preliminary evidence that human brain macrophages have a different oxygen tolerance compared to systemic macrophages and propose improvements to our in vitro system to make it applicable for data collection that aim at refining oxygen-guided therapy for patients with traumatic brain injury.

Original languageEnglish (US)
Title of host publication49th Annual Rocky Mountain Bioengineering Symposium and 49th International ISA Biomedical Sciences Instrumentation Symposium
Pages47-54
Number of pages8
StatePublished - Aug 20 2012
Event49th Annual Rocky Mountain Bioengineering Symposium and 49th International ISA Biomedical Sciences Instrumentation Symposium - Blacksburg, VA, United States
Duration: Mar 22 2012Mar 24 2012

Publication series

Name49th Annual Rocky Mountain Bioengineering Symposium and 49th International ISA Biomedical Sciences Instrumentation Symposium

Other

Other49th Annual Rocky Mountain Bioengineering Symposium and 49th International ISA Biomedical Sciences Instrumentation Symposium
CountryUnited States
CityBlacksburg, VA
Period3/22/123/24/12

Keywords

  • Emergency care
  • Hypoxia
  • Microglia
  • Modeling
  • Oxygen sensors
  • Polarography
  • Translational research

ASJC Scopus subject areas

  • Biomedical Engineering

Fingerprint Dive into the research topics of 'Measurements of low oxygen tension in vitro and response of macrophages to levels applicable to peri-and postoperative treatment of traumatic brain injury'. Together they form a unique fingerprint.

  • Cite this

    Waite, G. N., Egot-Lemaire, S., Bouwens, M., Owegi, H. O., Narotam, P., & Waite, L. R. (2012). Measurements of low oxygen tension in vitro and response of macrophages to levels applicable to peri-and postoperative treatment of traumatic brain injury. In 49th Annual Rocky Mountain Bioengineering Symposium and 49th International ISA Biomedical Sciences Instrumentation Symposium (pp. 47-54). (49th Annual Rocky Mountain Bioengineering Symposium and 49th International ISA Biomedical Sciences Instrumentation Symposium).