Mechanical stimulation suppresses phosphorylation of eIF2α and PERK-mediated responses to stress to the endoplasmic reticulum

Hideyuki Hirasawa, Chang Jiang, Ping Zhang, Feng Chun Yang, Hiroki Yokota

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Cellular perturbations such as stress to the endoplasmic reticulum induce an integrated stress response, which activates phosphorylation of eIF2α and leads to alleviation of cellular injury or apoptosis. This study investigated the role of mechanical stimulation in the regulation of eIF2α and cell death. Mechanical stimulation was applied to mouse ulnae, MC3T3 cells, and mesenchymal stem cells. The results demonstrate that mechanical stimulation reduces phosphorylation of eIF2α through inactivation of Perk. Furthermore, flow pre-treatment reduces thapsigargin-induced cell mortality through suppression of phosphorylation of Perk. However, H2O2-driven cell mortality, which is not mediated by Perk, is not suppressed by mechanical stimulation. Taken together, our observations suggest a pro-survival role of mechanical stimulation in Perk-mediated stress responses.

Original languageEnglish
Pages (from-to)745-752
Number of pages8
JournalFEBS Letters
Volume584
Issue number4
DOIs
StatePublished - Feb 2010

Fingerprint

Phosphorylation
Endoplasmic Reticulum Stress
Ulna
Thapsigargin
Mortality
Cell death
Stem cells
Mesenchymal Stromal Cells
Cell Death
Apoptosis
Wounds and Injuries

Keywords

  • Endoplasmic reticulum
  • Mechanical stimulation
  • MSC
  • Osteoblast
  • Perk
  • Ulna

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Structural Biology

Cite this

Mechanical stimulation suppresses phosphorylation of eIF2α and PERK-mediated responses to stress to the endoplasmic reticulum. / Hirasawa, Hideyuki; Jiang, Chang; Zhang, Ping; Yang, Feng Chun; Yokota, Hiroki.

In: FEBS Letters, Vol. 584, No. 4, 02.2010, p. 745-752.

Research output: Contribution to journalArticle

Hirasawa, Hideyuki ; Jiang, Chang ; Zhang, Ping ; Yang, Feng Chun ; Yokota, Hiroki. / Mechanical stimulation suppresses phosphorylation of eIF2α and PERK-mediated responses to stress to the endoplasmic reticulum. In: FEBS Letters. 2010 ; Vol. 584, No. 4. pp. 745-752.
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