Mechanism for initiation of food allergy: Dependence on skin barrier mutations and environmental allergen costimulation

Matthew T. Walker, Jeremy E. Green, Ryan P. Ferrie, Ashley M. Queener, Mark H. Kaplan, Joan M. Cook-Mills

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

Background: Mechanisms for the development of food allergy in neonates are unknown but clearly linked in patient populations to a genetic predisposition to skin barrier defects. Whether skin barrier defects contribute functionally to development of food allergy is unknown. Objective: The purpose of the study was to determine whether skin barrier mutations, which are primarily heterozygous in patient populations, contribute to the development of food allergy. Methods: Mice heterozygous for the filaggrin (Flg) ft and Tmem79 ma mutations were skin sensitized with environmental and food allergens. After sensitization, mice received oral challenge with food allergen, and then inflammation, inflammatory mediators, and anaphylaxis were measured. Results: We define development of inflammation, inflammatory mediators, and food allergen–induced anaphylaxis in neonatal mice with skin barrier mutations after brief concurrent cutaneous exposure to food and environmental allergens. Moreover, neonates of allergic mothers have increased responses to suboptimal sensitization with food allergens. Importantly, responses to food allergens by these neonatal mice were dependent on genetic defects in skin barrier function and on exposure to environmental allergens. ST2 blockade during skin sensitization inhibited the development of anaphylaxis, antigen-specific IgE, and inflammatory mediators. Neonatal anaphylactic responses and antigen-specific IgE were also inhibited by oral pre-exposure to food allergen, but interestingly, this was blunted by concurrent pre-exposure of the skin to environmental allergen. Conclusion: These studies uncover mechanisms for food allergy sensitization and anaphylaxis in neonatal mice that are consistent with features of human early-life exposures and genetics in patients with clinical food allergy and demonstrate that changes in barrier function drive development of anaphylaxis to food allergen.

Original languageEnglish (US)
Pages (from-to)1711-1725.e9
JournalJournal of Allergy and Clinical Immunology
Volume141
Issue number5
DOIs
StatePublished - May 2018

Keywords

  • Alternaria alternata
  • ST2
  • Sensitization
  • anaphylaxis
  • chicken egg ovalbumin
  • filaggrin
  • food allergy
  • house dust mite
  • mattrin
  • peanut
  • skin

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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