Mechanism mediating nitric oxide - Induced inhibition in human jejunal longitudinal smooth muscle

Nicholas Zyromski, Judith A. Duenes, Michael L. Kendrick, Bruno M. Balsiger, Gianrico Farrugia, Michael G. Sarr

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Background. Enteric neurotransmission is a complex process involving multiple neurotransmitters, including nitric oxide (NO). Our aim was to evaluate the role and mechanism(s) of action of NO in normal human jejunal longitudinal smooth muscle. Methods. Transmural strips of normal human jejunum obtained from subjects undergoing gastric bypass were studied in organ chambers. Effects of exogenous NO (7 × 10-6 mol/L to 7 × 10-5 mol/L) and electrical field stimulation (nonspecific release of endogenous neurotransmitters) on spontaneous contractile activity and on precontracted muscle strips (substance P, 10-5 mol/L) were evaluated in the presence and absence of the competitive NO synthase inhibitor NG-amino-L-arginine (L-NNA, 10-3 mol/L) and the specific soluble guanylyl cyclase inhibitor 1H-[1,2,4]-oxadiazaolo-[4,3-a]-quinoxalin-1-one (ODQ 10-5 mol/L and 10-4 mol/L). Results. Exogenous NO dose-dependently inhibited spontaneous contractility and relaxed precontracted smooth muscle strips. The effects of NO were markedly attenuated or completely inhibited in the presence of ODQ. Electric field stimulation under nonadrenergic, noncholinergic conditions also inhibited spontaneous contractility and relaxed precontracted smooth muscle strips; both of these effects were attenuated, but not completely inhibited, in the presence of both ODQ and L-NNA. Conclusions. NO is an endogenous inhibitory neurotransmitter in human jejunal longitudinal smooth muscle, acting at least in part via a mechanism mediated by guanylyl cyclase. Other (non-nitrergic) nonadrenergic, noncholinergic inhibitory neurotransmitters are likely active in this portion of the human gut.

Original languageEnglish (US)
Pages (from-to)489-496
Number of pages8
JournalSurgery
Volume130
Issue number3
DOIs
StatePublished - 2001
Externally publishedYes

Fingerprint

Smooth Muscle
Nitric Oxide
Neurotransmitter Agents
Electric Stimulation
Quinoxalines
Gastric Bypass
Guanylate Cyclase
Jejunum
Substance P
Nitric Oxide Synthase
Synaptic Transmission
Muscles

ASJC Scopus subject areas

  • Surgery

Cite this

Zyromski, N., Duenes, J. A., Kendrick, M. L., Balsiger, B. M., Farrugia, G., & Sarr, M. G. (2001). Mechanism mediating nitric oxide - Induced inhibition in human jejunal longitudinal smooth muscle. Surgery, 130(3), 489-496. https://doi.org/10.1067/msy.2001.116414

Mechanism mediating nitric oxide - Induced inhibition in human jejunal longitudinal smooth muscle. / Zyromski, Nicholas; Duenes, Judith A.; Kendrick, Michael L.; Balsiger, Bruno M.; Farrugia, Gianrico; Sarr, Michael G.

In: Surgery, Vol. 130, No. 3, 2001, p. 489-496.

Research output: Contribution to journalArticle

Zyromski, N, Duenes, JA, Kendrick, ML, Balsiger, BM, Farrugia, G & Sarr, MG 2001, 'Mechanism mediating nitric oxide - Induced inhibition in human jejunal longitudinal smooth muscle', Surgery, vol. 130, no. 3, pp. 489-496. https://doi.org/10.1067/msy.2001.116414
Zyromski, Nicholas ; Duenes, Judith A. ; Kendrick, Michael L. ; Balsiger, Bruno M. ; Farrugia, Gianrico ; Sarr, Michael G. / Mechanism mediating nitric oxide - Induced inhibition in human jejunal longitudinal smooth muscle. In: Surgery. 2001 ; Vol. 130, No. 3. pp. 489-496.
@article{9a67a0af818242e78c9815d9a3c5e969,
title = "Mechanism mediating nitric oxide - Induced inhibition in human jejunal longitudinal smooth muscle",
abstract = "Background. Enteric neurotransmission is a complex process involving multiple neurotransmitters, including nitric oxide (NO). Our aim was to evaluate the role and mechanism(s) of action of NO in normal human jejunal longitudinal smooth muscle. Methods. Transmural strips of normal human jejunum obtained from subjects undergoing gastric bypass were studied in organ chambers. Effects of exogenous NO (7 × 10-6 mol/L to 7 × 10-5 mol/L) and electrical field stimulation (nonspecific release of endogenous neurotransmitters) on spontaneous contractile activity and on precontracted muscle strips (substance P, 10-5 mol/L) were evaluated in the presence and absence of the competitive NO synthase inhibitor NG-amino-L-arginine (L-NNA, 10-3 mol/L) and the specific soluble guanylyl cyclase inhibitor 1H-[1,2,4]-oxadiazaolo-[4,3-a]-quinoxalin-1-one (ODQ 10-5 mol/L and 10-4 mol/L). Results. Exogenous NO dose-dependently inhibited spontaneous contractility and relaxed precontracted smooth muscle strips. The effects of NO were markedly attenuated or completely inhibited in the presence of ODQ. Electric field stimulation under nonadrenergic, noncholinergic conditions also inhibited spontaneous contractility and relaxed precontracted smooth muscle strips; both of these effects were attenuated, but not completely inhibited, in the presence of both ODQ and L-NNA. Conclusions. NO is an endogenous inhibitory neurotransmitter in human jejunal longitudinal smooth muscle, acting at least in part via a mechanism mediated by guanylyl cyclase. Other (non-nitrergic) nonadrenergic, noncholinergic inhibitory neurotransmitters are likely active in this portion of the human gut.",
author = "Nicholas Zyromski and Duenes, {Judith A.} and Kendrick, {Michael L.} and Balsiger, {Bruno M.} and Gianrico Farrugia and Sarr, {Michael G.}",
year = "2001",
doi = "10.1067/msy.2001.116414",
language = "English (US)",
volume = "130",
pages = "489--496",
journal = "Surgery",
issn = "0039-6060",
publisher = "Mosby Inc.",
number = "3",

}

TY - JOUR

T1 - Mechanism mediating nitric oxide - Induced inhibition in human jejunal longitudinal smooth muscle

AU - Zyromski, Nicholas

AU - Duenes, Judith A.

AU - Kendrick, Michael L.

AU - Balsiger, Bruno M.

AU - Farrugia, Gianrico

AU - Sarr, Michael G.

PY - 2001

Y1 - 2001

N2 - Background. Enteric neurotransmission is a complex process involving multiple neurotransmitters, including nitric oxide (NO). Our aim was to evaluate the role and mechanism(s) of action of NO in normal human jejunal longitudinal smooth muscle. Methods. Transmural strips of normal human jejunum obtained from subjects undergoing gastric bypass were studied in organ chambers. Effects of exogenous NO (7 × 10-6 mol/L to 7 × 10-5 mol/L) and electrical field stimulation (nonspecific release of endogenous neurotransmitters) on spontaneous contractile activity and on precontracted muscle strips (substance P, 10-5 mol/L) were evaluated in the presence and absence of the competitive NO synthase inhibitor NG-amino-L-arginine (L-NNA, 10-3 mol/L) and the specific soluble guanylyl cyclase inhibitor 1H-[1,2,4]-oxadiazaolo-[4,3-a]-quinoxalin-1-one (ODQ 10-5 mol/L and 10-4 mol/L). Results. Exogenous NO dose-dependently inhibited spontaneous contractility and relaxed precontracted smooth muscle strips. The effects of NO were markedly attenuated or completely inhibited in the presence of ODQ. Electric field stimulation under nonadrenergic, noncholinergic conditions also inhibited spontaneous contractility and relaxed precontracted smooth muscle strips; both of these effects were attenuated, but not completely inhibited, in the presence of both ODQ and L-NNA. Conclusions. NO is an endogenous inhibitory neurotransmitter in human jejunal longitudinal smooth muscle, acting at least in part via a mechanism mediated by guanylyl cyclase. Other (non-nitrergic) nonadrenergic, noncholinergic inhibitory neurotransmitters are likely active in this portion of the human gut.

AB - Background. Enteric neurotransmission is a complex process involving multiple neurotransmitters, including nitric oxide (NO). Our aim was to evaluate the role and mechanism(s) of action of NO in normal human jejunal longitudinal smooth muscle. Methods. Transmural strips of normal human jejunum obtained from subjects undergoing gastric bypass were studied in organ chambers. Effects of exogenous NO (7 × 10-6 mol/L to 7 × 10-5 mol/L) and electrical field stimulation (nonspecific release of endogenous neurotransmitters) on spontaneous contractile activity and on precontracted muscle strips (substance P, 10-5 mol/L) were evaluated in the presence and absence of the competitive NO synthase inhibitor NG-amino-L-arginine (L-NNA, 10-3 mol/L) and the specific soluble guanylyl cyclase inhibitor 1H-[1,2,4]-oxadiazaolo-[4,3-a]-quinoxalin-1-one (ODQ 10-5 mol/L and 10-4 mol/L). Results. Exogenous NO dose-dependently inhibited spontaneous contractility and relaxed precontracted smooth muscle strips. The effects of NO were markedly attenuated or completely inhibited in the presence of ODQ. Electric field stimulation under nonadrenergic, noncholinergic conditions also inhibited spontaneous contractility and relaxed precontracted smooth muscle strips; both of these effects were attenuated, but not completely inhibited, in the presence of both ODQ and L-NNA. Conclusions. NO is an endogenous inhibitory neurotransmitter in human jejunal longitudinal smooth muscle, acting at least in part via a mechanism mediated by guanylyl cyclase. Other (non-nitrergic) nonadrenergic, noncholinergic inhibitory neurotransmitters are likely active in this portion of the human gut.

UR - http://www.scopus.com/inward/record.url?scp=0034821651&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034821651&partnerID=8YFLogxK

U2 - 10.1067/msy.2001.116414

DO - 10.1067/msy.2001.116414

M3 - Article

C2 - 11562674

AN - SCOPUS:0034821651

VL - 130

SP - 489

EP - 496

JO - Surgery

JF - Surgery

SN - 0039-6060

IS - 3

ER -