Mechanism of action of the papillomavirus E2 represser: Repression in the absence of DNA binding

James Barsoum, S. S. Prakash, Paula Han, Elliot Androphy

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Repression of papillomavirus E2-dependent gene expression was studied by using transient transfections into mouse embryo fibroblast cells. Cotransfection of a gene corresponding to the naturally occurring represser E2-TR along with the full-length E2 gene resulted in up to 98% repression of E2-dependent reporter gene expression. A series of E2 DNA-binding domain mutants were transferred into the E2-TR form and characterized for their ability to repress E2-dependent transactivation. All mutants which were defective for DNA binding but were dimerization competent repressed E2 transactivation as well or nearly as well as the wild-type represser. E2 mutants which lacked dimerization activity repressed transactivation poorly or not at all. These results indicate that the E2 represser can inhibit transcription, in the absence of DNA binding, by forming heterodimers with full-length E2.

Original languageEnglish (US)
Pages (from-to)3941-3945
Number of pages5
JournalJournal of Virology
Volume66
Issue number6
StatePublished - Jun 1992
Externally publishedYes

Fingerprint

Papillomaviridae
transcriptional activation
Transcriptional Activation
mechanism of action
dimerization
Dimerization
mutants
DNA
Gene Expression
gene expression
DNA-binding domains
transfection
Reporter Genes
reporter genes
Genes
fibroblasts
Transfection
genes
Embryonic Structures
transcription (genetics)

ASJC Scopus subject areas

  • Immunology

Cite this

Mechanism of action of the papillomavirus E2 represser : Repression in the absence of DNA binding. / Barsoum, James; Prakash, S. S.; Han, Paula; Androphy, Elliot.

In: Journal of Virology, Vol. 66, No. 6, 06.1992, p. 3941-3945.

Research output: Contribution to journalArticle

Barsoum, James ; Prakash, S. S. ; Han, Paula ; Androphy, Elliot. / Mechanism of action of the papillomavirus E2 represser : Repression in the absence of DNA binding. In: Journal of Virology. 1992 ; Vol. 66, No. 6. pp. 3941-3945.
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