Mechanism of bicarbonate secretion in rat (Rattus rattus) colon

Pierre Dagher, John I. Rho, Alan N. Charney

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

1. 1. Colonie HCO3 secretion was measured as the residual flux in male Sprague-Dawley rats (Rattus rattus). 2. 2. Basal HCO3 secretion was increased by 1 mM dibutyryl cyclic AMP (dbcAMP) and was reduced to baseline by 0.1 mM methazolamide (Mtz) but not by SITS (1 mM), DIDS (1 mM) or amiloride (1 mM). 3. 3. In vivo, intravenous vasoactive intestinal peptide increased HCO3 secretion and prior perfusion with 1 mM Mtz prevented this increase. 4. 4. These results suggest that the source of basal and cAMP-stimulated HCO3 secretion is, in part, intracellular and requires the action of carbonic anhydrase.

Original languageEnglish (US)
Pages (from-to)43-48
Number of pages6
JournalComparative Biochemistry and Physiology -- Part A: Physiology
Volume105
Issue number1
DOIs
StatePublished - 1993
Externally publishedYes

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Methazolamide
Bicarbonates
Rats
Colon
4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid
4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid
Bucladesine
Carbonic Anhydrases
Amiloride
Vasoactive Intestinal Peptide
Sprague Dawley Rats
Perfusion
Fluxes

ASJC Scopus subject areas

  • Physiology
  • Molecular Biology
  • Biochemistry

Cite this

Mechanism of bicarbonate secretion in rat (Rattus rattus) colon. / Dagher, Pierre; Rho, John I.; Charney, Alan N.

In: Comparative Biochemistry and Physiology -- Part A: Physiology, Vol. 105, No. 1, 1993, p. 43-48.

Research output: Contribution to journalArticle

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