Mechanism of human natural killer cell activation by haemophilus ducreyi

Wei Li, Diane Janowicz, Kate R. Fortney, Barry Katz, Stanley Spinola

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The role of natural killer (NK) cells in the host response to Haemophilus ducreyi infection is unclear. In pustules obtained from infected human volunteers, there was an enrichment of CD56bright NK cells bearing the activation markers CD69 and HLA-DR, compared with peripheral blood. To study the mechanism by which H. ducreyi activated NK cells, we used peripheral blood mononuclear cells from uninfected volunteers. H. ducreyi activated NK cells only in the presence of antigen-presenting cells. H. ducreyi-infected monocytes and monocyte-derived macrophages activated NK cells in a contact- and interleukin-18 (IL-18)-dependent manner, whereas monocyte-derived dendritic cells induced NK activation through soluble IL-12. More lesionai NK cells than peripheral blood NK cells produced IFN-γ in response to IL-12 and IL-18. We conclude that NK cells are recruited to experimental lesions and likely are activated by infected macrophages and dendritic cells. IFN-γ produced by lesional NK cells may facilitate phagocytosis of H. ducreyi.

Original languageEnglish
Pages (from-to)590-598
Number of pages9
JournalJournal of Infectious Diseases
Volume200
Issue number4
DOIs
StatePublished - Aug 15 2009

Fingerprint

Haemophilus ducreyi
Natural Killer Cells
Interleukin-18
Interleukin-12
Dendritic Cells
Monocytes
Volunteers
Blood Cells
Haemophilus Infections
Macrophages
HLA-DR Antigens
Antigen-Presenting Cells
Phagocytosis

ASJC Scopus subject areas

  • Infectious Diseases
  • Immunology and Allergy

Cite this

Mechanism of human natural killer cell activation by haemophilus ducreyi. / Li, Wei; Janowicz, Diane; Fortney, Kate R.; Katz, Barry; Spinola, Stanley.

In: Journal of Infectious Diseases, Vol. 200, No. 4, 15.08.2009, p. 590-598.

Research output: Contribution to journalArticle

@article{647df45c3d304cb891ad2bb2d2358fcf,
title = "Mechanism of human natural killer cell activation by haemophilus ducreyi",
abstract = "The role of natural killer (NK) cells in the host response to Haemophilus ducreyi infection is unclear. In pustules obtained from infected human volunteers, there was an enrichment of CD56bright NK cells bearing the activation markers CD69 and HLA-DR, compared with peripheral blood. To study the mechanism by which H. ducreyi activated NK cells, we used peripheral blood mononuclear cells from uninfected volunteers. H. ducreyi activated NK cells only in the presence of antigen-presenting cells. H. ducreyi-infected monocytes and monocyte-derived macrophages activated NK cells in a contact- and interleukin-18 (IL-18)-dependent manner, whereas monocyte-derived dendritic cells induced NK activation through soluble IL-12. More lesionai NK cells than peripheral blood NK cells produced IFN-γ in response to IL-12 and IL-18. We conclude that NK cells are recruited to experimental lesions and likely are activated by infected macrophages and dendritic cells. IFN-γ produced by lesional NK cells may facilitate phagocytosis of H. ducreyi.",
author = "Wei Li and Diane Janowicz and Fortney, {Kate R.} and Barry Katz and Stanley Spinola",
year = "2009",
month = "8",
day = "15",
doi = "10.1086/600123",
language = "English",
volume = "200",
pages = "590--598",
journal = "Journal of Infectious Diseases",
issn = "0022-1899",
publisher = "Oxford University Press",
number = "4",

}

TY - JOUR

T1 - Mechanism of human natural killer cell activation by haemophilus ducreyi

AU - Li, Wei

AU - Janowicz, Diane

AU - Fortney, Kate R.

AU - Katz, Barry

AU - Spinola, Stanley

PY - 2009/8/15

Y1 - 2009/8/15

N2 - The role of natural killer (NK) cells in the host response to Haemophilus ducreyi infection is unclear. In pustules obtained from infected human volunteers, there was an enrichment of CD56bright NK cells bearing the activation markers CD69 and HLA-DR, compared with peripheral blood. To study the mechanism by which H. ducreyi activated NK cells, we used peripheral blood mononuclear cells from uninfected volunteers. H. ducreyi activated NK cells only in the presence of antigen-presenting cells. H. ducreyi-infected monocytes and monocyte-derived macrophages activated NK cells in a contact- and interleukin-18 (IL-18)-dependent manner, whereas monocyte-derived dendritic cells induced NK activation through soluble IL-12. More lesionai NK cells than peripheral blood NK cells produced IFN-γ in response to IL-12 and IL-18. We conclude that NK cells are recruited to experimental lesions and likely are activated by infected macrophages and dendritic cells. IFN-γ produced by lesional NK cells may facilitate phagocytosis of H. ducreyi.

AB - The role of natural killer (NK) cells in the host response to Haemophilus ducreyi infection is unclear. In pustules obtained from infected human volunteers, there was an enrichment of CD56bright NK cells bearing the activation markers CD69 and HLA-DR, compared with peripheral blood. To study the mechanism by which H. ducreyi activated NK cells, we used peripheral blood mononuclear cells from uninfected volunteers. H. ducreyi activated NK cells only in the presence of antigen-presenting cells. H. ducreyi-infected monocytes and monocyte-derived macrophages activated NK cells in a contact- and interleukin-18 (IL-18)-dependent manner, whereas monocyte-derived dendritic cells induced NK activation through soluble IL-12. More lesionai NK cells than peripheral blood NK cells produced IFN-γ in response to IL-12 and IL-18. We conclude that NK cells are recruited to experimental lesions and likely are activated by infected macrophages and dendritic cells. IFN-γ produced by lesional NK cells may facilitate phagocytosis of H. ducreyi.

UR - http://www.scopus.com/inward/record.url?scp=69149094927&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=69149094927&partnerID=8YFLogxK

U2 - 10.1086/600123

DO - 10.1086/600123

M3 - Article

C2 - 19572804

AN - SCOPUS:69149094927

VL - 200

SP - 590

EP - 598

JO - Journal of Infectious Diseases

JF - Journal of Infectious Diseases

SN - 0022-1899

IS - 4

ER -