Mechanism of immunopotentiation by aluminum-containing adjuvants elucidated by the relationship between antigen retention at the inoculation site and the immune response

Stephanie M. Noe, Mark A. Green, Harm HogenEsch, Stanley L. Hem

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59 Scopus citations


The relationship between depot formation and immunopotentiation was studied by comparing the retention of antigen at the inoculation site with antibody production in rats. A model 111In-labeled alpha casein (IDCAS) antigen was formulated into four vaccines: IDCAS adsorbed onto either aluminum hydroxide adjuvant (AH) or aluminum phosphate adjuvant (AP); non-adsorbed IDCAS with phosphate-treated AP (PTAP); and IDCAS solution. Gamma scintigraphy showed the order of retention following subcutaneous administration to be: AH adsorbed > AP adsorbed > non-adsorbed with PTAP = solution. The antibody titers followed the order: non-adsorbed with PTAP = AP adsorbed > AH adsorbed ≫ solution. The presence of an aluminum-containing adjuvant was essential for immunopotentiation, but retention of the antigen at the inoculation site was not required.

Original languageEnglish (US)
Pages (from-to)3588-3594
Number of pages7
Issue number20
StatePublished - Apr 30 2010



  • Aluminum-containing adjuvants
  • Immunopotentiation
  • Retention at inoculation site

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

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