Mechanisms of biphasic insulin-granule exocytosis - Roles of the cytoskeleton, small GTPases and SNARE proteins

Zhanxiang Wang, Debbie C. Thurmond

Research output: Contribution to journalArticle

202 Citations (Scopus)

Abstract

The release of insulin from pancreatic islets requires negative regulation to ensure low levels of insulin release under resting conditions, as well as positive regulation to facilitate robust responsiveness to conditions of elevated fuel or glucose. The first phase of release involves the plasma-membrane fusion of a small pool of granules, termed the readily releasable pool; these granules are already at the membrane under basal conditions, and discharge their cargo in response to nutrient and also non-nutrient secretagogues. By contrast, second-phase secretion is evoked exclusively by nutrients, and involves the mobilization of intracellular granules to t-SNARE sites at the plasma membrane to enable the distal docking and fusion steps of insulin exocytosis. Nearly 40 years ago, the actin cytoskeleton was first recognized as a key mediator of biphasic insulin release, and was originally presumed to act as a barrier to block granule docking at the cell periphery. More recently, however, the discovery of cycling GTPases that are involved in F-actin reorganization in the islet β-cell, combined with the availability of reagents that are more specific and tools with which to study the mechanisms that underlie granule movement, have contributed greatly to our understanding of the role of the cytoskeleton in regulating biphasic insulin secretion. Herein, we provide historical perspective and review recent progress that has been made towards integrating cytoskeletal reorganization and cycling of small Rho-, Rab- and Ras-family GTPases into our current models of stimulus-secretion coupling and second-phase insulin release.

Original languageEnglish
Pages (from-to)893-903
Number of pages11
JournalJournal of Cell Science
Volume122
Issue number7
DOIs
StatePublished - Apr 1 2009

Fingerprint

Biphasic Insulins
SNARE Proteins
Monomeric GTP-Binding Proteins
Exocytosis
Cytoskeleton
Insulin
Islets of Langerhans
Cell Membrane
ras Proteins
Food
Membrane Fusion
GTP Phosphohydrolases
Actin Cytoskeleton
Actins
Glucose
Membranes

Keywords

  • Biphasic insulin secretion
  • F-actin reorganization
  • Islet
  • Microtubule
  • Small GTPases
  • SNARE protein

ASJC Scopus subject areas

  • Cell Biology

Cite this

Mechanisms of biphasic insulin-granule exocytosis - Roles of the cytoskeleton, small GTPases and SNARE proteins. / Wang, Zhanxiang; Thurmond, Debbie C.

In: Journal of Cell Science, Vol. 122, No. 7, 01.04.2009, p. 893-903.

Research output: Contribution to journalArticle

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