Mechanisms of leukocyte production and release. XII. A comparative assay of the leukocytosis inducing factor (LIF) and the colony stimulating factor (CSF)

Hal Broxmeyer, G. van Zant, J. R. Zucali, J. LoBue, A. S. Gordon

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Experiments are reported which suggest that separate physiologic regulators exist for neutrophil production and release. Sera obtained 2 hr post iv injection of 4.0 μg E. coli lipopolysaccharide contained both leukocytosis inducing factor (LIF) and colony stimulating factor (CSF) activity, whereas sera obtained 27 hr after the last of 2 and the last of 5 daily ip injections of 100.0 μg E. coli lipopolysaccharide demonstrated only LIF activity. Using the Etoxate test, it was established that neither LIF nor CSF activity could be attributed to endotoxin contamination of test sera.

Original languageEnglish (US)
Pages (from-to)1262-1267
Number of pages6
JournalProceedings of the Society for Experimental Biology and Medicine
Volume145
Issue number4
StatePublished - 1974
Externally publishedYes

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Colony-Stimulating Factors
Assays
Leukocytes
Escherichia coli
Lipopolysaccharides
Serum
Injections
Endotoxins
Neutrophils
Contamination
leukocyte mobilizing factor
Experiments

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

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title = "Mechanisms of leukocyte production and release. XII. A comparative assay of the leukocytosis inducing factor (LIF) and the colony stimulating factor (CSF)",
abstract = "Experiments are reported which suggest that separate physiologic regulators exist for neutrophil production and release. Sera obtained 2 hr post iv injection of 4.0 μg E. coli lipopolysaccharide contained both leukocytosis inducing factor (LIF) and colony stimulating factor (CSF) activity, whereas sera obtained 27 hr after the last of 2 and the last of 5 daily ip injections of 100.0 μg E. coli lipopolysaccharide demonstrated only LIF activity. Using the Etoxate test, it was established that neither LIF nor CSF activity could be attributed to endotoxin contamination of test sera.",
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year = "1974",
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TY - JOUR

T1 - Mechanisms of leukocyte production and release. XII. A comparative assay of the leukocytosis inducing factor (LIF) and the colony stimulating factor (CSF)

AU - Broxmeyer, Hal

AU - van Zant, G.

AU - Zucali, J. R.

AU - LoBue, J.

AU - Gordon, A. S.

PY - 1974

Y1 - 1974

N2 - Experiments are reported which suggest that separate physiologic regulators exist for neutrophil production and release. Sera obtained 2 hr post iv injection of 4.0 μg E. coli lipopolysaccharide contained both leukocytosis inducing factor (LIF) and colony stimulating factor (CSF) activity, whereas sera obtained 27 hr after the last of 2 and the last of 5 daily ip injections of 100.0 μg E. coli lipopolysaccharide demonstrated only LIF activity. Using the Etoxate test, it was established that neither LIF nor CSF activity could be attributed to endotoxin contamination of test sera.

AB - Experiments are reported which suggest that separate physiologic regulators exist for neutrophil production and release. Sera obtained 2 hr post iv injection of 4.0 μg E. coli lipopolysaccharide contained both leukocytosis inducing factor (LIF) and colony stimulating factor (CSF) activity, whereas sera obtained 27 hr after the last of 2 and the last of 5 daily ip injections of 100.0 μg E. coli lipopolysaccharide demonstrated only LIF activity. Using the Etoxate test, it was established that neither LIF nor CSF activity could be attributed to endotoxin contamination of test sera.

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VL - 145

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EP - 1267

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