Mechanisms of the release of anterogradely transported neurotrophin-3 from axon terminals

Xiaoxia Wang, Rafal Butowt, Michael Vasko, Christopher S. Von Bartheld

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Neurotrophins have profound effects on synaptic function and structure. They can be derived from presynaptic, as well as postsynaptic, sites. To date, it has not been possible to measure the release of neurotrophins from axon terminals in intact tissue. We implemented a novel, extremely sensitive assay for the release and transfer of anterogradely transported neurotrophin-3 (NT-3) from a presynaptic to a postsynaptic location that uses synaptosomal fractionation after introduction of radiolabeled NT-3 into the retinotectal projection of chick embryos. Release of the anterogradely transported NT-3 in intact tissue was assessed by measuring the amount remaining in synaptosomal preparations after treatment of whole tecta with pharmacological agents. Use of this assay reveals that release of NT-3 from axon terminals is increased by depolarization, calcium influx via N-type calcium channels, and cAMP analogs, and release is most profoundly increased by excitation with kainic acid or mobilization of calcium from intracellular stores. NT-3 release depends on extracellular sodium, CaM kinase II activity, and requires intact microtubules and microfilaments. Dantrolene inhibits the high potassium-induced release of NT-3, indicating that release of calcium from intracellular stores is required. Tetanus toxin also inhibits NT-3 release, suggesting that intact synaptobrevin or synaptobrevin-like molecules are required for exocytosis. Ultrastructural autoradiography and immunolabel indicate that NT-3 is packaged in presumptive large dense-core vesicles. These data show that release of NT-3 from axon terminals depends on multiple regulatory proteins and ions, including the mobilization of local calcium. The data provide insight in the mechanisms of anterograde neurotrophins as synaptic modulators.

Original languageEnglish
Pages (from-to)931-945
Number of pages15
JournalJournal of Neuroscience
Volume22
Issue number3
StatePublished - Feb 1 2002

Fingerprint

Neurotrophin 3
Presynaptic Terminals
Nerve Growth Factors
R-SNARE Proteins
Calcium
N-Type Calcium Channels
Dantrolene
Tetanus Toxin
Calcium-Calmodulin-Dependent Protein Kinase Type 2
Kainic Acid
Exocytosis
Secretory Vesicles
Chick Embryo
Autoradiography
Actin Cytoskeleton
Microtubules
Potassium
Sodium
Pharmacology
Ions

Keywords

  • Axonal transport
  • Calcium
  • Neurotrophic factors
  • Neurotrophin
  • Presynaptic terminals
  • Secretion
  • Synapse
  • Synaptic transmission

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Mechanisms of the release of anterogradely transported neurotrophin-3 from axon terminals. / Wang, Xiaoxia; Butowt, Rafal; Vasko, Michael; Von Bartheld, Christopher S.

In: Journal of Neuroscience, Vol. 22, No. 3, 01.02.2002, p. 931-945.

Research output: Contribution to journalArticle

Wang, Xiaoxia ; Butowt, Rafal ; Vasko, Michael ; Von Bartheld, Christopher S. / Mechanisms of the release of anterogradely transported neurotrophin-3 from axon terminals. In: Journal of Neuroscience. 2002 ; Vol. 22, No. 3. pp. 931-945.
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