Mediator and SAGA Have Distinct Roles in Pol II Preinitiation Complex Assembly and Function

Xiao Fen Chen, Lynn Lehmann, Justin J. Lin, Ajay Vashisht, Ryan Schmidt, Roberto Ferrari, Chengyang Huang, Robin McKee, Amber Mosley, Kathrin Plath, Siavash K. Kurdistani, James Wohlschlegel, Michael Carey

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

A key feature of RNA polymerase II (Pol II) preinitiation complexes (PICs) is their ability to coordinate transcription initiation with chromatin modification and remodeling. To understand how this coordination is achieved, we employed extensive proteomic and mechanistic analyses to study the composition and assembly of PICs in HeLa cell and mouse embryonic stem cell (ESC) nuclear extracts. Strikingly, most of the machinery that is necessary for transcription initiation on chromatin is part of the PIC. The PIC is nearly identical between ESCs and HeLa cells and contains two major coactivator complexes: Mediator and SAGA. Genome-wide analysis of Mediator reveals that it has a close correlation with Pol II, TATA-binding protein, and messenger RNA levels and thus may play a major role in PIC assembly. Moreover, Mediator coordinates assembly of the Pol II initiation factors and chromatin machinery into a PIC in vitro, whereas SAGA acts after PIC assembly to allow transcription on chromatin.

Original languageEnglish
Pages (from-to)1061-1067
Number of pages7
JournalCell Reports
Volume2
Issue number5
DOIs
StatePublished - Nov 29 2012

Fingerprint

Chromatin
Transcription
HeLa Cells
Mediator Complex
TATA-Box Binding Protein
Machinery
Peptide Initiation Factors
Chromatin Assembly and Disassembly
RNA Polymerase II
Cell Extracts
Proteomics
Stem cells
Genome
Messenger RNA
Genes
Chemical analysis
In Vitro Techniques
Mouse Embryonic Stem Cells

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Chen, X. F., Lehmann, L., Lin, J. J., Vashisht, A., Schmidt, R., Ferrari, R., ... Carey, M. (2012). Mediator and SAGA Have Distinct Roles in Pol II Preinitiation Complex Assembly and Function. Cell Reports, 2(5), 1061-1067. https://doi.org/10.1016/j.celrep.2012.10.019

Mediator and SAGA Have Distinct Roles in Pol II Preinitiation Complex Assembly and Function. / Chen, Xiao Fen; Lehmann, Lynn; Lin, Justin J.; Vashisht, Ajay; Schmidt, Ryan; Ferrari, Roberto; Huang, Chengyang; McKee, Robin; Mosley, Amber; Plath, Kathrin; Kurdistani, Siavash K.; Wohlschlegel, James; Carey, Michael.

In: Cell Reports, Vol. 2, No. 5, 29.11.2012, p. 1061-1067.

Research output: Contribution to journalArticle

Chen, XF, Lehmann, L, Lin, JJ, Vashisht, A, Schmidt, R, Ferrari, R, Huang, C, McKee, R, Mosley, A, Plath, K, Kurdistani, SK, Wohlschlegel, J & Carey, M 2012, 'Mediator and SAGA Have Distinct Roles in Pol II Preinitiation Complex Assembly and Function', Cell Reports, vol. 2, no. 5, pp. 1061-1067. https://doi.org/10.1016/j.celrep.2012.10.019
Chen XF, Lehmann L, Lin JJ, Vashisht A, Schmidt R, Ferrari R et al. Mediator and SAGA Have Distinct Roles in Pol II Preinitiation Complex Assembly and Function. Cell Reports. 2012 Nov 29;2(5):1061-1067. https://doi.org/10.1016/j.celrep.2012.10.019
Chen, Xiao Fen ; Lehmann, Lynn ; Lin, Justin J. ; Vashisht, Ajay ; Schmidt, Ryan ; Ferrari, Roberto ; Huang, Chengyang ; McKee, Robin ; Mosley, Amber ; Plath, Kathrin ; Kurdistani, Siavash K. ; Wohlschlegel, James ; Carey, Michael. / Mediator and SAGA Have Distinct Roles in Pol II Preinitiation Complex Assembly and Function. In: Cell Reports. 2012 ; Vol. 2, No. 5. pp. 1061-1067.
@article{8afc95f0ec2d4a1c987b1df65d47c748,
title = "Mediator and SAGA Have Distinct Roles in Pol II Preinitiation Complex Assembly and Function",
abstract = "A key feature of RNA polymerase II (Pol II) preinitiation complexes (PICs) is their ability to coordinate transcription initiation with chromatin modification and remodeling. To understand how this coordination is achieved, we employed extensive proteomic and mechanistic analyses to study the composition and assembly of PICs in HeLa cell and mouse embryonic stem cell (ESC) nuclear extracts. Strikingly, most of the machinery that is necessary for transcription initiation on chromatin is part of the PIC. The PIC is nearly identical between ESCs and HeLa cells and contains two major coactivator complexes: Mediator and SAGA. Genome-wide analysis of Mediator reveals that it has a close correlation with Pol II, TATA-binding protein, and messenger RNA levels and thus may play a major role in PIC assembly. Moreover, Mediator coordinates assembly of the Pol II initiation factors and chromatin machinery into a PIC in vitro, whereas SAGA acts after PIC assembly to allow transcription on chromatin.",
author = "Chen, {Xiao Fen} and Lynn Lehmann and Lin, {Justin J.} and Ajay Vashisht and Ryan Schmidt and Roberto Ferrari and Chengyang Huang and Robin McKee and Amber Mosley and Kathrin Plath and Kurdistani, {Siavash K.} and James Wohlschlegel and Michael Carey",
year = "2012",
month = "11",
day = "29",
doi = "10.1016/j.celrep.2012.10.019",
language = "English",
volume = "2",
pages = "1061--1067",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Cell Press",
number = "5",

}

TY - JOUR

T1 - Mediator and SAGA Have Distinct Roles in Pol II Preinitiation Complex Assembly and Function

AU - Chen, Xiao Fen

AU - Lehmann, Lynn

AU - Lin, Justin J.

AU - Vashisht, Ajay

AU - Schmidt, Ryan

AU - Ferrari, Roberto

AU - Huang, Chengyang

AU - McKee, Robin

AU - Mosley, Amber

AU - Plath, Kathrin

AU - Kurdistani, Siavash K.

AU - Wohlschlegel, James

AU - Carey, Michael

PY - 2012/11/29

Y1 - 2012/11/29

N2 - A key feature of RNA polymerase II (Pol II) preinitiation complexes (PICs) is their ability to coordinate transcription initiation with chromatin modification and remodeling. To understand how this coordination is achieved, we employed extensive proteomic and mechanistic analyses to study the composition and assembly of PICs in HeLa cell and mouse embryonic stem cell (ESC) nuclear extracts. Strikingly, most of the machinery that is necessary for transcription initiation on chromatin is part of the PIC. The PIC is nearly identical between ESCs and HeLa cells and contains two major coactivator complexes: Mediator and SAGA. Genome-wide analysis of Mediator reveals that it has a close correlation with Pol II, TATA-binding protein, and messenger RNA levels and thus may play a major role in PIC assembly. Moreover, Mediator coordinates assembly of the Pol II initiation factors and chromatin machinery into a PIC in vitro, whereas SAGA acts after PIC assembly to allow transcription on chromatin.

AB - A key feature of RNA polymerase II (Pol II) preinitiation complexes (PICs) is their ability to coordinate transcription initiation with chromatin modification and remodeling. To understand how this coordination is achieved, we employed extensive proteomic and mechanistic analyses to study the composition and assembly of PICs in HeLa cell and mouse embryonic stem cell (ESC) nuclear extracts. Strikingly, most of the machinery that is necessary for transcription initiation on chromatin is part of the PIC. The PIC is nearly identical between ESCs and HeLa cells and contains two major coactivator complexes: Mediator and SAGA. Genome-wide analysis of Mediator reveals that it has a close correlation with Pol II, TATA-binding protein, and messenger RNA levels and thus may play a major role in PIC assembly. Moreover, Mediator coordinates assembly of the Pol II initiation factors and chromatin machinery into a PIC in vitro, whereas SAGA acts after PIC assembly to allow transcription on chromatin.

UR - http://www.scopus.com/inward/record.url?scp=84870433555&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84870433555&partnerID=8YFLogxK

U2 - 10.1016/j.celrep.2012.10.019

DO - 10.1016/j.celrep.2012.10.019

M3 - Article

VL - 2

SP - 1061

EP - 1067

JO - Cell Reports

JF - Cell Reports

SN - 2211-1247

IS - 5

ER -