Mediator Head module structure and functional interactions

Gang Cai, Tsuyoshi Imasaki, Kentaro Yamada, Francesco Cardelli, Yuichiro Takagi, Francisco J. Asturias

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

We used single-particle electron microscopy to characterize the structure and subunit organization of the Mediator Head module that controls Mediator-RNA polymerase II (RNAPII) and Mediator-promoter interactions. The Head module adopts several conformations differing in the position of a movable jaw formed by the Med18-Med20 subcomplex. We also characterized, by structural, biochemical and genetic means, the interactions of the Head module with TATA-binding protein (TBP) and RNAPII subunits Rpb4 and Rpb7. TBP binds near the Med18-Med20 attachment point and stabilizes an open conformation of the Head module. Rpb4 and Rpb7 bind between the Head jaws, establishing contacts essential for yeast-cell viability. These results, and consideration of the structure of the Mediator-RNAPII holoenzyme, shed light on the stabilization of the pre-initiation complex by Mediator and suggest how Mediator might influence initiation by modulating polymerase conformation and interaction with promoter DNA.

Original languageEnglish
Pages (from-to)273-279
Number of pages7
JournalNature Structural and Molecular Biology
Volume17
Issue number3
DOIs
StatePublished - Mar 2010

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Head
TATA-Box Binding Protein
RNA Polymerase II
Jaw
Mediator Complex
Holoenzymes
Molecular Biology
Cell Survival
Electron Microscopy
Yeasts
DNA

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

Cite this

Mediator Head module structure and functional interactions. / Cai, Gang; Imasaki, Tsuyoshi; Yamada, Kentaro; Cardelli, Francesco; Takagi, Yuichiro; Asturias, Francisco J.

In: Nature Structural and Molecular Biology, Vol. 17, No. 3, 03.2010, p. 273-279.

Research output: Contribution to journalArticle

Cai, G, Imasaki, T, Yamada, K, Cardelli, F, Takagi, Y & Asturias, FJ 2010, 'Mediator Head module structure and functional interactions', Nature Structural and Molecular Biology, vol. 17, no. 3, pp. 273-279. https://doi.org/10.1038/nsmb.1757
Cai, Gang ; Imasaki, Tsuyoshi ; Yamada, Kentaro ; Cardelli, Francesco ; Takagi, Yuichiro ; Asturias, Francisco J. / Mediator Head module structure and functional interactions. In: Nature Structural and Molecular Biology. 2010 ; Vol. 17, No. 3. pp. 273-279.
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