Megakaryocyte-bone cell interactions

Melissa Kacena, Wendy A. Ciovacco

Research output: Chapter in Book/Report/Conference proceedingConference contribution

11 Citations (Scopus)

Abstract

Emerging data show that megakaryocytes MKs) play a role in the replication and development of bone cells. Both in vivo and in vitro evidence now show that MKs can have significant effects on cells of the osteoclast OC) and osteoblast OB) lineage with obvious manifestations on bone phenotype and probable significance for human pathology. There are currently four mouse models in which increases in MK number lead to a specific bone pathology of markedly increased bone volume. While these models all achieve megakaryocytosis by different mechanisms the resultant osteosclerotic phenotype observed is consistent across all models. In vitro data suggest that MKs play a role in OC and OB proliferation and differentiation. While MKs express receptor activator of nuclear factor kappa B ligand (RANKL) a prerequisite for osteoclastogenesis they also express many factors known to inhibit OC development and co-cultures of MKs with OCs show a significant decrease in osteoclastogenesis. In contrast MKs express several proteins with a known critical role in osteoblastogenesis and bone formation and co-cultures of these two lineages result in up to a six-fold increase in OB proliferation and alterations in OB differentiation. This research demonstrates the complex regulatory interactions at play between MKs and bone cells and opens up potential targets for therapeutic intervention.

Original languageEnglish
Title of host publicationAdvances in Experimental Medicine and Biology
Pages31-41
Number of pages11
Volume658
DOIs
StatePublished - 2010

Publication series

NameAdvances in Experimental Medicine and Biology
Volume658
ISSN (Print)00652598

Fingerprint

Megakaryocytes
Cell Communication
Bone
Osteogenesis
Bone and Bones
Coculture Techniques
Pathology
RANK Ligand
Phenotype
Bone Development
Osteoclasts
Osteoblasts
Research
Proteins
In Vitro Techniques
Therapeutics

Keywords

  • Bone
  • GATA-1
  • Megakaryocyte
  • NFE2
  • Osteoblast
  • OsteoClast
  • TPO
  • Von Willenbrand

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Kacena, M., & Ciovacco, W. A. (2010). Megakaryocyte-bone cell interactions. In Advances in Experimental Medicine and Biology (Vol. 658, pp. 31-41). (Advances in Experimental Medicine and Biology; Vol. 658). https://doi.org/10.1007/978-1-4419-1050-9_4

Megakaryocyte-bone cell interactions. / Kacena, Melissa; Ciovacco, Wendy A.

Advances in Experimental Medicine and Biology. Vol. 658 2010. p. 31-41 (Advances in Experimental Medicine and Biology; Vol. 658).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Kacena, M & Ciovacco, WA 2010, Megakaryocyte-bone cell interactions. in Advances in Experimental Medicine and Biology. vol. 658, Advances in Experimental Medicine and Biology, vol. 658, pp. 31-41. https://doi.org/10.1007/978-1-4419-1050-9_4
Kacena M, Ciovacco WA. Megakaryocyte-bone cell interactions. In Advances in Experimental Medicine and Biology. Vol. 658. 2010. p. 31-41. (Advances in Experimental Medicine and Biology). https://doi.org/10.1007/978-1-4419-1050-9_4
Kacena, Melissa ; Ciovacco, Wendy A. / Megakaryocyte-bone cell interactions. Advances in Experimental Medicine and Biology. Vol. 658 2010. pp. 31-41 (Advances in Experimental Medicine and Biology).
@inproceedings{3bbff8e355af4a6a9d2dea16269089af,
title = "Megakaryocyte-bone cell interactions",
abstract = "Emerging data show that megakaryocytes MKs) play a role in the replication and development of bone cells. Both in vivo and in vitro evidence now show that MKs can have significant effects on cells of the osteoclast OC) and osteoblast OB) lineage with obvious manifestations on bone phenotype and probable significance for human pathology. There are currently four mouse models in which increases in MK number lead to a specific bone pathology of markedly increased bone volume. While these models all achieve megakaryocytosis by different mechanisms the resultant osteosclerotic phenotype observed is consistent across all models. In vitro data suggest that MKs play a role in OC and OB proliferation and differentiation. While MKs express receptor activator of nuclear factor kappa B ligand (RANKL) a prerequisite for osteoclastogenesis they also express many factors known to inhibit OC development and co-cultures of MKs with OCs show a significant decrease in osteoclastogenesis. In contrast MKs express several proteins with a known critical role in osteoblastogenesis and bone formation and co-cultures of these two lineages result in up to a six-fold increase in OB proliferation and alterations in OB differentiation. This research demonstrates the complex regulatory interactions at play between MKs and bone cells and opens up potential targets for therapeutic intervention.",
keywords = "Bone, GATA-1, Megakaryocyte, NFE2, Osteoblast, OsteoClast, TPO, Von Willenbrand",
author = "Melissa Kacena and Ciovacco, {Wendy A.}",
year = "2010",
doi = "10.1007/978-1-4419-1050-9_4",
language = "English",
isbn = "9781441910493",
volume = "658",
series = "Advances in Experimental Medicine and Biology",
pages = "31--41",
booktitle = "Advances in Experimental Medicine and Biology",

}

TY - GEN

T1 - Megakaryocyte-bone cell interactions

AU - Kacena, Melissa

AU - Ciovacco, Wendy A.

PY - 2010

Y1 - 2010

N2 - Emerging data show that megakaryocytes MKs) play a role in the replication and development of bone cells. Both in vivo and in vitro evidence now show that MKs can have significant effects on cells of the osteoclast OC) and osteoblast OB) lineage with obvious manifestations on bone phenotype and probable significance for human pathology. There are currently four mouse models in which increases in MK number lead to a specific bone pathology of markedly increased bone volume. While these models all achieve megakaryocytosis by different mechanisms the resultant osteosclerotic phenotype observed is consistent across all models. In vitro data suggest that MKs play a role in OC and OB proliferation and differentiation. While MKs express receptor activator of nuclear factor kappa B ligand (RANKL) a prerequisite for osteoclastogenesis they also express many factors known to inhibit OC development and co-cultures of MKs with OCs show a significant decrease in osteoclastogenesis. In contrast MKs express several proteins with a known critical role in osteoblastogenesis and bone formation and co-cultures of these two lineages result in up to a six-fold increase in OB proliferation and alterations in OB differentiation. This research demonstrates the complex regulatory interactions at play between MKs and bone cells and opens up potential targets for therapeutic intervention.

AB - Emerging data show that megakaryocytes MKs) play a role in the replication and development of bone cells. Both in vivo and in vitro evidence now show that MKs can have significant effects on cells of the osteoclast OC) and osteoblast OB) lineage with obvious manifestations on bone phenotype and probable significance for human pathology. There are currently four mouse models in which increases in MK number lead to a specific bone pathology of markedly increased bone volume. While these models all achieve megakaryocytosis by different mechanisms the resultant osteosclerotic phenotype observed is consistent across all models. In vitro data suggest that MKs play a role in OC and OB proliferation and differentiation. While MKs express receptor activator of nuclear factor kappa B ligand (RANKL) a prerequisite for osteoclastogenesis they also express many factors known to inhibit OC development and co-cultures of MKs with OCs show a significant decrease in osteoclastogenesis. In contrast MKs express several proteins with a known critical role in osteoblastogenesis and bone formation and co-cultures of these two lineages result in up to a six-fold increase in OB proliferation and alterations in OB differentiation. This research demonstrates the complex regulatory interactions at play between MKs and bone cells and opens up potential targets for therapeutic intervention.

KW - Bone

KW - GATA-1

KW - Megakaryocyte

KW - NFE2

KW - Osteoblast

KW - OsteoClast

KW - TPO

KW - Von Willenbrand

UR - http://www.scopus.com/inward/record.url?scp=78149415448&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78149415448&partnerID=8YFLogxK

U2 - 10.1007/978-1-4419-1050-9_4

DO - 10.1007/978-1-4419-1050-9_4

M3 - Conference contribution

C2 - 19950013

AN - SCOPUS:78149415448

SN - 9781441910493

VL - 658

T3 - Advances in Experimental Medicine and Biology

SP - 31

EP - 41

BT - Advances in Experimental Medicine and Biology

ER -