Megakaryocyte-mediated inhibition of osteoclast development

Melissa Kacena, Tracy Nelson, Mary E. Clough, Sun Kyeong Lee, Joseph A. Lorenzo, Caren M. Gundberg, Mark C. Horowitz

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

A growing body of evidence indicates that megakaryocytes (MK) or their growth factors play a role in skeletal homeostasis. We previously identified a novel regulatory pathway that controls bone formation, which is mediated by MK. In vivo megakaryocytosis resulted in massive bone formation. The co-culture of MK with osteoblasts (OB) resulted in increased OB proliferation in vitro, by a mechanism that required direct cell-to-cell contact. Here, we examined a second MK-mediated pathway that regulates osteoclast (OC) development. We have begun examining the unique inhibitory effect of MK on OC development. Spleen or bone marrow (BM) cells from C57BL/6 mice, as a source of OC precursors, were cultured with M-CSF and RANKL to induce OC development. MK were prepared by culturing fetal liver cells with thrombopoietin and separating cells into MK and non-MK populations. MK were titrated into spleen cell cultures and OC were identified as tartrate-resistant acid phosphatase-positive giant cells with >3 nuclei. There was a significant, P 

Original languageEnglish (US)
Pages (from-to)991-999
Number of pages9
JournalBone
Volume39
Issue number5
DOIs
StatePublished - Nov 2006
Externally publishedYes

Fingerprint

Megakaryocytes
Osteoclasts
Osteoblasts
Osteogenesis
Spleen
Thrombopoietin
Macrophage Colony-Stimulating Factor
Giant Cells
Coculture Techniques
Inbred C57BL Mouse
Bone Marrow Cells
Intercellular Signaling Peptides and Proteins
Homeostasis
Cell Culture Techniques
Liver

Keywords

  • Cytokine
  • Megakaryocyte
  • OPG
  • Osteoclast inhibition

ASJC Scopus subject areas

  • Physiology
  • Hematology

Cite this

Kacena, M., Nelson, T., Clough, M. E., Lee, S. K., Lorenzo, J. A., Gundberg, C. M., & Horowitz, M. C. (2006). Megakaryocyte-mediated inhibition of osteoclast development. Bone, 39(5), 991-999. https://doi.org/10.1016/j.bone.2006.05.004

Megakaryocyte-mediated inhibition of osteoclast development. / Kacena, Melissa; Nelson, Tracy; Clough, Mary E.; Lee, Sun Kyeong; Lorenzo, Joseph A.; Gundberg, Caren M.; Horowitz, Mark C.

In: Bone, Vol. 39, No. 5, 11.2006, p. 991-999.

Research output: Contribution to journalArticle

Kacena, M, Nelson, T, Clough, ME, Lee, SK, Lorenzo, JA, Gundberg, CM & Horowitz, MC 2006, 'Megakaryocyte-mediated inhibition of osteoclast development', Bone, vol. 39, no. 5, pp. 991-999. https://doi.org/10.1016/j.bone.2006.05.004
Kacena M, Nelson T, Clough ME, Lee SK, Lorenzo JA, Gundberg CM et al. Megakaryocyte-mediated inhibition of osteoclast development. Bone. 2006 Nov;39(5):991-999. https://doi.org/10.1016/j.bone.2006.05.004
Kacena, Melissa ; Nelson, Tracy ; Clough, Mary E. ; Lee, Sun Kyeong ; Lorenzo, Joseph A. ; Gundberg, Caren M. ; Horowitz, Mark C. / Megakaryocyte-mediated inhibition of osteoclast development. In: Bone. 2006 ; Vol. 39, No. 5. pp. 991-999.
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