Melanocyte mitogens induce both melanocyte chemokinesis and chemotaxis

T. Horikawa, D. A. Norris, J. J. Yohn, T. Zekman, Jeffrey Travers, J. G. Morelli

Research output: Contribution to journalArticle

87 Citations (Scopus)

Abstract

It is believed that during repigmentation of vitiligo, inactive melanocytes in the outer root sheath of the hair follicle become activated, proliferate, and migrate into the depigmented skin. However, the mechanisms controlling melanocyte migration remain to be elucidated. In this study, we investigated the effects of well-described melanocyte growth factors on melanocyte migration. Using time-lapse photography, we demonstrated that melanocyte chemokinetic movement was induced by basic fibroblast growth factor, stem cell factor, and endothelin-1, with the greatest effect noted using 100 nM endothelin-1. Similar results were reported previously with leukotriene C4. When surrounded by these stimuli, melanocytes moved in a random, nonlinear fashion and showed no desensitization at the concentrations studied. In Boyden chamber checkerboard analysis, basic fibroblast growth factor, leukotriene C4 and endothelin-1 were chemotactic. They produced directional migration and showed desensitization at higher concentrations. The greatest effect again was seen with 100 nM endothelin-1. Stem cell factor showed no effect in this assay system at the concentrations tested. The four melanocyte mitogens - leukotriene C4, endothelin-1, basic fibroblast growth factor, and stem cell factor stimulate melanocyte migration, and this migration may be either chemokinetic (activated random movement) or chemotactic (requiring a gradient, directional, and slowing desensitization), depending on the conditions used. We believe that these factors may be effective in stimulating vitiligo repigmentation by inducing proliferation and migration of hair-follicle outer-root-sheath melanocytes into the depigmented epidermis.

Original languageEnglish (US)
Pages (from-to)256-259
Number of pages4
JournalJournal of Investigative Dermatology
Volume104
Issue number2
StatePublished - 1995
Externally publishedYes

Fingerprint

Melanocytes
Endothelin-1
Chemotaxis
Mitogens
Leukotriene C4
Stem Cell Factor
Fibroblast Growth Factor 2
Vitiligo
Special effects
Hair Follicle
Assays
Intercellular Signaling Peptides and Proteins
Skin
Photography
Epidermis

Keywords

  • Growth factors
  • Melanocyte movement
  • Repigmentation
  • Vitiligo

ASJC Scopus subject areas

  • Dermatology

Cite this

Horikawa, T., Norris, D. A., Yohn, J. J., Zekman, T., Travers, J., & Morelli, J. G. (1995). Melanocyte mitogens induce both melanocyte chemokinesis and chemotaxis. Journal of Investigative Dermatology, 104(2), 256-259.

Melanocyte mitogens induce both melanocyte chemokinesis and chemotaxis. / Horikawa, T.; Norris, D. A.; Yohn, J. J.; Zekman, T.; Travers, Jeffrey; Morelli, J. G.

In: Journal of Investigative Dermatology, Vol. 104, No. 2, 1995, p. 256-259.

Research output: Contribution to journalArticle

Horikawa, T, Norris, DA, Yohn, JJ, Zekman, T, Travers, J & Morelli, JG 1995, 'Melanocyte mitogens induce both melanocyte chemokinesis and chemotaxis', Journal of Investigative Dermatology, vol. 104, no. 2, pp. 256-259.
Horikawa T, Norris DA, Yohn JJ, Zekman T, Travers J, Morelli JG. Melanocyte mitogens induce both melanocyte chemokinesis and chemotaxis. Journal of Investigative Dermatology. 1995;104(2):256-259.
Horikawa, T. ; Norris, D. A. ; Yohn, J. J. ; Zekman, T. ; Travers, Jeffrey ; Morelli, J. G. / Melanocyte mitogens induce both melanocyte chemokinesis and chemotaxis. In: Journal of Investigative Dermatology. 1995 ; Vol. 104, No. 2. pp. 256-259.
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