Membrane binding and bending in Ebola VP40 assembly and egress

Research output: Contribution to journalReview article

40 Scopus citations

Abstract

Lipid-enveloped viruses contain a lipid bilayer coat that protects their genome and helps to facilitate entry into the host cell. Filoviruses are lipid-enveloped viruses that have up to 90% clinical fatality and include Marbug (MARV) and Ebola (EBOV). These pleomorphic filamentous viruses enter the host cell through their membrane-embedded glycoprotein and then replicate using just seven genes encoded in their negative-sense RNA genome. EBOV budding occurs from the inner leaflet of the plasma membrane (PM) and is driven by the matrix protein VP40, which is the most abundantly expressed protein of the virus. VP40 expressed in mammalian cells alone can trigger budding of filamentous virus-like particles (VLPs) that are nearly indistinguishable from authentic EBOV. VP40, such as matrix proteins from other viruses, has been shown to bind anionic lipid membranes. However, how VP40 selectively interacts with the inner leaflet of the PM and assembles into a filamentous lipid enveloped particle is mostly unknown. This article describes what is known regarding VP40 membrane interactions and what answers will fill the gaps.

Original languageEnglish (US)
Article number300
JournalFrontiers in Microbiology
Volume5
Issue numberJUN
DOIs
StatePublished - Jan 1 2014

Keywords

  • Ebola
  • Filovirus
  • Membrane binding
  • Phosphatidylserine
  • Phosphoinositides
  • Plasma membrane
  • VP40

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

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