Membranous expression of glucose transporter-1 protein (GLUT-1) in embryonal neoplasms of the central nervous system

M. Loda, X. Xu, A. Pession, Alexander Vortmeyer, F. Giangaspero

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The human erythrocyte GLUT-1 is a transmembrane protein which facilitates transport of glucose in the cell in an energy-independent fashion. Neuroectodermal stem cells show strong membrane immunoreactivitry with this marker at early developmental stages in rodents. Membranous expression by undifferentiated neuroectodermal cells gradually decreases while GLUT-1 becomes confined to the endothelial cells, when these acquire blood-brain barrier function. We thus sought to determine whether GLUT-1 expression was limited to embryonal neoplasms of the central nervous system (CNS) which are presumably derived from developmentally arrested neuroectodermal stem cells. Archival material of 40 primary CNS neoplasms were examined for immunoreactivity with anti-GLUT-1. This included both non-embryonal neoplasms (18 astrocytic tumours, one ependymoma and three oligodendroglioma) and embryonal neoplasms (12 cerebellar medulloblastomas, four supratentorial PNETs and two atypical teratoid/rhabdoid tumours (AT/RhT)). In addition, cell lines and nude mice xenografts derived from both undifferentiated and differentiated tumours were assessed for GLUT-1 immunoreactivity by both immunohistochemistry and Western blotting. All embryonal tumours, MBs and PNET xenografts consistently showed GLUT-1 membrane staining. Non-embryonal neoplasms were negative except for vascular staining. Membrane protein fraction of embryonal tumours cell lines immunoreacted by immunoblot with GLUT-1, whereas the glioblastoma cell line was negative. Expression of GLUT-1 supports the stem cell nature of the cells of origin of MBs, supratentorial PNET and AT/RhTs. As a result, GLUT-1 is a useful marker to define the embryonal nature of CNS neoplasms.

Original languageEnglish (US)
Pages (from-to)91-97
Number of pages7
JournalNeuropathology and Applied Neurobiology
Volume26
Issue number1
DOIs
StatePublished - Mar 23 2000
Externally publishedYes

Fingerprint

Facilitative Glucose Transport Proteins
Germ Cell and Embryonal Neoplasms
Central Nervous System
Primitive Neuroectodermal Tumors
Proteins
Central Nervous System Neoplasms
Stem Cells
Neoplasms
Heterografts
Membrane Proteins
Glucose Transporter Type 1
Staining and Labeling
Oligodendroglioma
Cell Line
Ependymoma
Medulloblastoma
Glioblastoma
Tumor Cell Line
Blood-Brain Barrier
Nude Mice

Keywords

  • Brain neoplasms
  • Embryonal neoplasms
  • Glucose transporter protein
  • Medulloblastoma
  • PNET

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Neurology
  • Clinical Neurology
  • Physiology (medical)

Cite this

Membranous expression of glucose transporter-1 protein (GLUT-1) in embryonal neoplasms of the central nervous system. / Loda, M.; Xu, X.; Pession, A.; Vortmeyer, Alexander; Giangaspero, F.

In: Neuropathology and Applied Neurobiology, Vol. 26, No. 1, 23.03.2000, p. 91-97.

Research output: Contribution to journalArticle

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