Mesencephalic dopamine cell deficit involves areas A8, A9 and A10 in weaver mutant mice

L. C. Triarhou, J. Norton, Bernardino Ghetti

Research output: Contribution to journalArticle

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Abstract

The mesencephalic dopamine (DA) cell system was examined in mice homozygous and heterozygous for the weaver (wv) gene and in wildtype controls to estimate the extent of cell losses associated with the genetically determined central DA deficiency observed in weaver homozygotes. Animals of the three genotypes (+/+, wv/+, wv/wv) were studied at postnatal day (P)20 and P90. Serial coronal sections were obtained through the brainstem. Half of the sections were immunolabeled with antiserum to tyrosine hydroxylase (TH). Cell counts were obtained in areas A8 (retrorubral nucleus, RRN), A9 (substantia nigra, SN), and A10 (ventral tegmental area, VTA). The counts were analyzed with repeated measures analysis of variance followed by individual comparisons among group means. In A8, weaver homozygotes did not differ significantly from wild-type controls at P20, whereas there was a significant difference of 56% at P90. In A9, weaver homozygotes differed significantly from wild-type mice by 42% at P20 and by 69% at P90. The decrease in cell number between P20 and P90 in weaver homozygotes was 54%. In A10, weaver homozygotes did not differ significantly from wild-type controls at P20, whereas there was a significant difference of 26% at P90. Cell numbers in all three areas of heterozygotes did not differ significantly from wildtype control values at either age point. These findings demonstrate that by three months of age homozygous weaver mutants exhibit nerve cell losses in all three areas of the mesencephalic DA cell system. Such losses account for the DA deficiency seen in striatal, limbic and cortical projection fields.

Original languageEnglish
Pages (from-to)256-265
Number of pages10
JournalExperimental Brain Research
Volume70
Issue number2
DOIs
StatePublished - Apr 1988

Fingerprint

Neurologic Mutant Mice
antineoplaston A10
Homozygote
Dopamine
Cell Count
Corpus Striatum
Ventral Tegmental Area
Tyrosine 3-Monooxygenase
Substantia Nigra
Heterozygote
Brain Stem
Immune Sera
Analysis of Variance
Genotype
Neurons
Genes

Keywords

  • Immunocytochemistry
  • Mesotelencephalic dopamine projection system
  • Morphometry
  • Retrorubral nucleus
  • Substantia nigra
  • Tyrosine hydroxylase
  • Ventral tegmental area
  • Weaver mutant mouse

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Mesencephalic dopamine cell deficit involves areas A8, A9 and A10 in weaver mutant mice. / Triarhou, L. C.; Norton, J.; Ghetti, Bernardino.

In: Experimental Brain Research, Vol. 70, No. 2, 04.1988, p. 256-265.

Research output: Contribution to journalArticle

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