Mesenchymal Stem Cells Attenuate Hypoxic Pulmonary Vasoconstriction by a Paracrine Mechanism

Ketan M. Patel, Paul Crisostomo, Tim Lahm, Troy Markel, Christine Herring, Meijing Wang, Kirstan K. Meldrum, Keith D. Lillemoe, Daniel R. Meldrum

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Background: Hypoxic pulmonary vasoconstriction (HPV) may be an adaptive mechanism to correct ventilation-perfusion mismatch in the face of hypoxia. In chronic hypoxia, prolonged vasoconstriction may result in pulmonary hypertension and cor pulmonale. It has been shown that during chronic hypoxia, mesenchymal stem cells (MSCs) may contribute to pulmonary vascular remodeling, anti-inflammation, and vascular stability. Also, MSCs have been shown to release growth factors when stressed by hypoxia. We hypothesized that MSCs reduce HPV by a paracrine mechanism. To test this, MSCs were stressed by hypoxia in tissue culture and the cell-free media was then used to treat the pulmonary arteries subjected to HPV. Methods: Adult male (250-350 g) Sprague Dawley rat pulmonary arteries (n = 10/group) were isolated and suspended in physiological organ baths. Human MSCs were stressed with 60-min hypoxia and conditioned media was collected. Pulmonary artery rings were treated with vehicle or MSC-conditioned (cell-free) media prior to hypoxia. Force displacement was continuously recorded. Data (mean ± SEM) were analyzed with two-way analysis of variance with post-hoc Bonferroni test. Results: Pulmonary arteries exposed to MSC-conditioned media experienced an augmented vasodilatory phase as compared to vehicle. Maximum vasodilation was 53.58 ± 6.42% versus 39.76 ± 4.05% for vehicle (P < 0.001). In addition, delayed, phase II vasoconstriction was significantly attenuated as compared to vehicle. Maximum phase II vasoconstriction was 28.51 ± 12.42 versus 86.29 ± 15.99% for vehicle (P < 0.001). Conclusions: We conclude that acute hypoxia induces HPV and that MSC-conditioned media acutely attenuates this effect. Thus, in addition to a direct contribution to vessel remodeling in chronic hypoxia, MSCs may acutely protect and attenuate hypoxic pulmonary vasoreactivity through a paracrine mechanism.

Original languageEnglish
Pages (from-to)281-285
Number of pages5
JournalJournal of Surgical Research
Volume143
Issue number2
DOIs
StatePublished - Dec 2007

Fingerprint

Vasoconstriction
Mesenchymal Stromal Cells
Lung
Pulmonary Artery
Conditioned Culture Medium
Hypoxia
Pulmonary Heart Disease
Baths
Pulmonary Hypertension
Vasodilation
Blood Vessels
Sprague Dawley Rats
Ventilation
Intercellular Signaling Peptides and Proteins
Analysis of Variance
Cell Culture Techniques
Perfusion
Inflammation

Keywords

  • conditioned media
  • hypoxic pulmonary vasoconstriction
  • mesenchymal stem cells
  • paracrine

ASJC Scopus subject areas

  • Surgery

Cite this

Mesenchymal Stem Cells Attenuate Hypoxic Pulmonary Vasoconstriction by a Paracrine Mechanism. / Patel, Ketan M.; Crisostomo, Paul; Lahm, Tim; Markel, Troy; Herring, Christine; Wang, Meijing; Meldrum, Kirstan K.; Lillemoe, Keith D.; Meldrum, Daniel R.

In: Journal of Surgical Research, Vol. 143, No. 2, 12.2007, p. 281-285.

Research output: Contribution to journalArticle

Patel, KM, Crisostomo, P, Lahm, T, Markel, T, Herring, C, Wang, M, Meldrum, KK, Lillemoe, KD & Meldrum, DR 2007, 'Mesenchymal Stem Cells Attenuate Hypoxic Pulmonary Vasoconstriction by a Paracrine Mechanism', Journal of Surgical Research, vol. 143, no. 2, pp. 281-285. https://doi.org/10.1016/j.jss.2006.11.006
Patel, Ketan M. ; Crisostomo, Paul ; Lahm, Tim ; Markel, Troy ; Herring, Christine ; Wang, Meijing ; Meldrum, Kirstan K. ; Lillemoe, Keith D. ; Meldrum, Daniel R. / Mesenchymal Stem Cells Attenuate Hypoxic Pulmonary Vasoconstriction by a Paracrine Mechanism. In: Journal of Surgical Research. 2007 ; Vol. 143, No. 2. pp. 281-285.
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abstract = "Background: Hypoxic pulmonary vasoconstriction (HPV) may be an adaptive mechanism to correct ventilation-perfusion mismatch in the face of hypoxia. In chronic hypoxia, prolonged vasoconstriction may result in pulmonary hypertension and cor pulmonale. It has been shown that during chronic hypoxia, mesenchymal stem cells (MSCs) may contribute to pulmonary vascular remodeling, anti-inflammation, and vascular stability. Also, MSCs have been shown to release growth factors when stressed by hypoxia. We hypothesized that MSCs reduce HPV by a paracrine mechanism. To test this, MSCs were stressed by hypoxia in tissue culture and the cell-free media was then used to treat the pulmonary arteries subjected to HPV. Methods: Adult male (250-350 g) Sprague Dawley rat pulmonary arteries (n = 10/group) were isolated and suspended in physiological organ baths. Human MSCs were stressed with 60-min hypoxia and conditioned media was collected. Pulmonary artery rings were treated with vehicle or MSC-conditioned (cell-free) media prior to hypoxia. Force displacement was continuously recorded. Data (mean ± SEM) were analyzed with two-way analysis of variance with post-hoc Bonferroni test. Results: Pulmonary arteries exposed to MSC-conditioned media experienced an augmented vasodilatory phase as compared to vehicle. Maximum vasodilation was 53.58 ± 6.42{\%} versus 39.76 ± 4.05{\%} for vehicle (P < 0.001). In addition, delayed, phase II vasoconstriction was significantly attenuated as compared to vehicle. Maximum phase II vasoconstriction was 28.51 ± 12.42 versus 86.29 ± 15.99{\%} for vehicle (P < 0.001). Conclusions: We conclude that acute hypoxia induces HPV and that MSC-conditioned media acutely attenuates this effect. Thus, in addition to a direct contribution to vessel remodeling in chronic hypoxia, MSCs may acutely protect and attenuate hypoxic pulmonary vasoreactivity through a paracrine mechanism.",
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AU - Crisostomo, Paul

AU - Lahm, Tim

AU - Markel, Troy

AU - Herring, Christine

AU - Wang, Meijing

AU - Meldrum, Kirstan K.

AU - Lillemoe, Keith D.

AU - Meldrum, Daniel R.

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N2 - Background: Hypoxic pulmonary vasoconstriction (HPV) may be an adaptive mechanism to correct ventilation-perfusion mismatch in the face of hypoxia. In chronic hypoxia, prolonged vasoconstriction may result in pulmonary hypertension and cor pulmonale. It has been shown that during chronic hypoxia, mesenchymal stem cells (MSCs) may contribute to pulmonary vascular remodeling, anti-inflammation, and vascular stability. Also, MSCs have been shown to release growth factors when stressed by hypoxia. We hypothesized that MSCs reduce HPV by a paracrine mechanism. To test this, MSCs were stressed by hypoxia in tissue culture and the cell-free media was then used to treat the pulmonary arteries subjected to HPV. Methods: Adult male (250-350 g) Sprague Dawley rat pulmonary arteries (n = 10/group) were isolated and suspended in physiological organ baths. Human MSCs were stressed with 60-min hypoxia and conditioned media was collected. Pulmonary artery rings were treated with vehicle or MSC-conditioned (cell-free) media prior to hypoxia. Force displacement was continuously recorded. Data (mean ± SEM) were analyzed with two-way analysis of variance with post-hoc Bonferroni test. Results: Pulmonary arteries exposed to MSC-conditioned media experienced an augmented vasodilatory phase as compared to vehicle. Maximum vasodilation was 53.58 ± 6.42% versus 39.76 ± 4.05% for vehicle (P < 0.001). In addition, delayed, phase II vasoconstriction was significantly attenuated as compared to vehicle. Maximum phase II vasoconstriction was 28.51 ± 12.42 versus 86.29 ± 15.99% for vehicle (P < 0.001). Conclusions: We conclude that acute hypoxia induces HPV and that MSC-conditioned media acutely attenuates this effect. Thus, in addition to a direct contribution to vessel remodeling in chronic hypoxia, MSCs may acutely protect and attenuate hypoxic pulmonary vasoreactivity through a paracrine mechanism.

AB - Background: Hypoxic pulmonary vasoconstriction (HPV) may be an adaptive mechanism to correct ventilation-perfusion mismatch in the face of hypoxia. In chronic hypoxia, prolonged vasoconstriction may result in pulmonary hypertension and cor pulmonale. It has been shown that during chronic hypoxia, mesenchymal stem cells (MSCs) may contribute to pulmonary vascular remodeling, anti-inflammation, and vascular stability. Also, MSCs have been shown to release growth factors when stressed by hypoxia. We hypothesized that MSCs reduce HPV by a paracrine mechanism. To test this, MSCs were stressed by hypoxia in tissue culture and the cell-free media was then used to treat the pulmonary arteries subjected to HPV. Methods: Adult male (250-350 g) Sprague Dawley rat pulmonary arteries (n = 10/group) were isolated and suspended in physiological organ baths. Human MSCs were stressed with 60-min hypoxia and conditioned media was collected. Pulmonary artery rings were treated with vehicle or MSC-conditioned (cell-free) media prior to hypoxia. Force displacement was continuously recorded. Data (mean ± SEM) were analyzed with two-way analysis of variance with post-hoc Bonferroni test. Results: Pulmonary arteries exposed to MSC-conditioned media experienced an augmented vasodilatory phase as compared to vehicle. Maximum vasodilation was 53.58 ± 6.42% versus 39.76 ± 4.05% for vehicle (P < 0.001). In addition, delayed, phase II vasoconstriction was significantly attenuated as compared to vehicle. Maximum phase II vasoconstriction was 28.51 ± 12.42 versus 86.29 ± 15.99% for vehicle (P < 0.001). Conclusions: We conclude that acute hypoxia induces HPV and that MSC-conditioned media acutely attenuates this effect. Thus, in addition to a direct contribution to vessel remodeling in chronic hypoxia, MSCs may acutely protect and attenuate hypoxic pulmonary vasoreactivity through a paracrine mechanism.

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