Metabolic effects of proglycosyn

Kunio Yamanouchi, Thomas W. Stephens, Kiwami Chikada, Samuel J. Dominianni, Heidi Behforouz, Peter Scislowski, Anna De Paoli-Roach, David W. Allmann, Robert Harris

Research output: Contribution to journalArticle

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Abstract

Proglycosyn, a phenylacyl imidazolium compound that lowers blood glucose levels, was demonstrated previously to promote hepatic glycogen synthesis, stabilize hepatic glycogen stores, activate glycogen synthase, inactivate glycogen phosphorylase, and inhibit glycolysis. In the present study proglycosyn was found to inhibit fatty acid synthesis, stimulate fatty acid oxidation, and lower fructose 2,6-bisphosphate levels, but to have no significant effects on cell swelling and the levels of cAMP in hepatocytes prepared from fed rats. Verapamil and atropine blocked the effects of proglycosyn on glycogen metabolism, but these compounds inhibit proglycosyn accumulation by hepatocytes. Proglycosyn stimulated phosphoprotein phosphatase activity in postmitochondrial extracts, as measured by dephosphorylation of phosphorylase a and glycogen synthase D, but this action required a very high concentration of the compound, making it unlikely to be the actual mechanism involved. It is proposed that a metabolite of proglycosyn is responsible for its metabolic effects.

Original languageEnglish
Pages (from-to)609-615
Number of pages7
JournalArchives of Biochemistry and Biophysics
Volume294
Issue number2
DOIs
StatePublished - May 1 1992

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proglycosyn
Glycogen Synthase
Liver Glycogen
Hepatocytes
Fatty Acids
Phosphorylase a
Glycogen Phosphorylase
Phosphoprotein Phosphatases
Glycolysis
Metabolites
Verapamil
Glycogen
Atropine
Metabolism
Swelling
Blood Glucose
Rats

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Yamanouchi, K., Stephens, T. W., Chikada, K., Dominianni, S. J., Behforouz, H., Scislowski, P., ... Harris, R. (1992). Metabolic effects of proglycosyn. Archives of Biochemistry and Biophysics, 294(2), 609-615. https://doi.org/10.1016/0003-9861(92)90732-C

Metabolic effects of proglycosyn. / Yamanouchi, Kunio; Stephens, Thomas W.; Chikada, Kiwami; Dominianni, Samuel J.; Behforouz, Heidi; Scislowski, Peter; De Paoli-Roach, Anna; Allmann, David W.; Harris, Robert.

In: Archives of Biochemistry and Biophysics, Vol. 294, No. 2, 01.05.1992, p. 609-615.

Research output: Contribution to journalArticle

Yamanouchi, K, Stephens, TW, Chikada, K, Dominianni, SJ, Behforouz, H, Scislowski, P, De Paoli-Roach, A, Allmann, DW & Harris, R 1992, 'Metabolic effects of proglycosyn', Archives of Biochemistry and Biophysics, vol. 294, no. 2, pp. 609-615. https://doi.org/10.1016/0003-9861(92)90732-C
Yamanouchi K, Stephens TW, Chikada K, Dominianni SJ, Behforouz H, Scislowski P et al. Metabolic effects of proglycosyn. Archives of Biochemistry and Biophysics. 1992 May 1;294(2):609-615. https://doi.org/10.1016/0003-9861(92)90732-C
Yamanouchi, Kunio ; Stephens, Thomas W. ; Chikada, Kiwami ; Dominianni, Samuel J. ; Behforouz, Heidi ; Scislowski, Peter ; De Paoli-Roach, Anna ; Allmann, David W. ; Harris, Robert. / Metabolic effects of proglycosyn. In: Archives of Biochemistry and Biophysics. 1992 ; Vol. 294, No. 2. pp. 609-615.
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