Metabolome Profiling of Partial and Fully Reprogrammed Induced Pluripotent Stem Cells

Soon Jung Park, Sang A. Lee, Nutan Prasain, Daekyeong Bae, Hyunsu Kang, Taewon Ha, Jong Soo Kim, Ki Sung Hong, Charlie Mantel, Sung Hwan Moon, Hal Broxmeyer, Man Ryul Lee

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Acquisition of proper metabolomic fate is required to convert somatic cells toward fully reprogrammed pluripotent stem cells. The majority of induced pluripotent stem cells (iPSCs) are partially reprogrammed and have a transcriptome different from that of the pluripotent stem cells. The metabolomic profile and mitochondrial metabolic functions required to achieve full reprogramming of somatic cells to iPSC status have not yet been elucidated. Clarification of the metabolites underlying reprogramming mechanisms should enable further optimization to enhance the efficiency of obtaining fully reprogrammed iPSCs. In this study, we characterized the metabolites of human fully reprogrammed iPSCs, partially reprogrammed iPSCs, and embryonic stem cells (ESCs). Using capillary electrophoresis time-of-flight mass spectrometry-based metabolomics, we found that 89% of analyzed metabolites were similarly expressed in fully reprogrammed iPSCs and human ESCs (hESCs), whereas partially reprogrammed iPSCs shared only 74% similarly expressed metabolites with hESCs. Metabolomic profiling analysis suggested that converting mitochondrial respiration to glycolytic flux is critical for reprogramming of somatic cells into fully reprogrammed iPSCs. This characterization of metabolic reprogramming in iPSCs may enable the development of new reprogramming parameters for enhancing the generation of fully reprogrammed human iPSCs.

Original languageEnglish (US)
Pages (from-to)734-742
Number of pages9
JournalStem Cells and Development
Volume26
Issue number10
DOIs
StatePublished - May 15 2017

Fingerprint

Induced Pluripotent Stem Cells
Metabolome
Metabolomics
Pluripotent Stem Cells
Capillary Electrophoresis
Embryonic Stem Cells
Transcriptome
Mass Spectrometry
Respiration

Keywords

  • Metabolism
  • Metabolomics
  • Oxidative phosphorylation
  • Reprogramming efficiency

ASJC Scopus subject areas

  • Hematology
  • Developmental Biology
  • Cell Biology

Cite this

Park, S. J., Lee, S. A., Prasain, N., Bae, D., Kang, H., Ha, T., ... Lee, M. R. (2017). Metabolome Profiling of Partial and Fully Reprogrammed Induced Pluripotent Stem Cells. Stem Cells and Development, 26(10), 734-742. https://doi.org/10.1089/scd.2016.0320

Metabolome Profiling of Partial and Fully Reprogrammed Induced Pluripotent Stem Cells. / Park, Soon Jung; Lee, Sang A.; Prasain, Nutan; Bae, Daekyeong; Kang, Hyunsu; Ha, Taewon; Kim, Jong Soo; Hong, Ki Sung; Mantel, Charlie; Moon, Sung Hwan; Broxmeyer, Hal; Lee, Man Ryul.

In: Stem Cells and Development, Vol. 26, No. 10, 15.05.2017, p. 734-742.

Research output: Contribution to journalArticle

Park, SJ, Lee, SA, Prasain, N, Bae, D, Kang, H, Ha, T, Kim, JS, Hong, KS, Mantel, C, Moon, SH, Broxmeyer, H & Lee, MR 2017, 'Metabolome Profiling of Partial and Fully Reprogrammed Induced Pluripotent Stem Cells', Stem Cells and Development, vol. 26, no. 10, pp. 734-742. https://doi.org/10.1089/scd.2016.0320
Park, Soon Jung ; Lee, Sang A. ; Prasain, Nutan ; Bae, Daekyeong ; Kang, Hyunsu ; Ha, Taewon ; Kim, Jong Soo ; Hong, Ki Sung ; Mantel, Charlie ; Moon, Sung Hwan ; Broxmeyer, Hal ; Lee, Man Ryul. / Metabolome Profiling of Partial and Fully Reprogrammed Induced Pluripotent Stem Cells. In: Stem Cells and Development. 2017 ; Vol. 26, No. 10. pp. 734-742.
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