Methods for microRNA profiling in cancer

Sushuma Yarlagadda, Anusha Thota, Ruchi Bansal, Jason Kwon, Murray Korc, Janaiah Kota

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

MicroRNAs (miRNA) are small non-coding RNAs that negatively regulate post-transcriptional gene expression. Almost all human cancers are characterized by abnormal microRNA expression patterns, which are unique to tumor types. A large body of experimental evidence documents the role of miRNAs in cancer pathogenesis, and specific miRNAs function as oncogenes or tumor suppressors. Due to unique expression profiles and anti/pro-tumorigenic properties of miRNAs, efforts are underway to explore their therapeutic and diagnostic potential. Many miRNA profiling methods have been developed, ranging from Northern blotting and qRT-PCR to the more recent microarray and RNA-Seq platforms. The following chapter details an imaging technique for cellular-specific miRNA expression profiling called in situ hybridization (ISH).

Original languageEnglish (US)
Title of host publicationMethods in Pharmacology and Toxicology
PublisherHumana Press Inc.
Pages97-113
Number of pages17
DOIs
StatePublished - 2017

Publication series

NameMethods in Pharmacology and Toxicology
ISSN (Print)15572153
ISSN (Electronic)19406053

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Keywords

  • Cancer
  • Expression profiling
  • ISH (in situ hybridization)
  • microRNA
  • Molecular imaging

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Pharmacology (medical)

Cite this

Yarlagadda, S., Thota, A., Bansal, R., Kwon, J., Korc, M., & Kota, J. (2017). Methods for microRNA profiling in cancer. In Methods in Pharmacology and Toxicology (pp. 97-113). (Methods in Pharmacology and Toxicology). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6743-8_7