Methods for microRNA profiling in cancer

Sushuma Yarlagadda, Anusha Thota, Ruchi Bansal, Jason Kwon, Murray Korc, Janaiah Kota

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

MicroRNAs (miRNA) are small non-coding RNAs that negatively regulate post-transcriptional gene expression. Almost all human cancers are characterized by abnormal microRNA expression patterns, which are unique to tumor types. A large body of experimental evidence documents the role of miRNAs in cancer pathogenesis, and specific miRNAs function as oncogenes or tumor suppressors. Due to unique expression profiles and anti/pro-tumorigenic properties of miRNAs, efforts are underway to explore their therapeutic and diagnostic potential. Many miRNA profiling methods have been developed, ranging from Northern blotting and qRT-PCR to the more recent microarray and RNA-Seq platforms. The following chapter details an imaging technique for cellular-specific miRNA expression profiling called in situ hybridization (ISH).

Original languageEnglish (US)
Title of host publicationMethods in Pharmacology and Toxicology
PublisherHumana Press Inc.
Pages97-113
Number of pages17
DOIs
StatePublished - 2017

Publication series

NameMethods in Pharmacology and Toxicology
ISSN (Print)15572153
ISSN (Electronic)19406053

Fingerprint

MicroRNAs
Neoplasms
Small Untranslated RNA
Oncogenes
Northern Blotting
In Situ Hybridization
RNA
Gene Expression
Polymerase Chain Reaction

Keywords

  • Cancer
  • Expression profiling
  • ISH (in situ hybridization)
  • microRNA
  • Molecular imaging

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Pharmacology (medical)

Cite this

Yarlagadda, S., Thota, A., Bansal, R., Kwon, J., Korc, M., & Kota, J. (2017). Methods for microRNA profiling in cancer. In Methods in Pharmacology and Toxicology (pp. 97-113). (Methods in Pharmacology and Toxicology). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6743-8_7

Methods for microRNA profiling in cancer. / Yarlagadda, Sushuma; Thota, Anusha; Bansal, Ruchi; Kwon, Jason; Korc, Murray; Kota, Janaiah.

Methods in Pharmacology and Toxicology. Humana Press Inc., 2017. p. 97-113 (Methods in Pharmacology and Toxicology).

Research output: Chapter in Book/Report/Conference proceedingChapter

Yarlagadda, S, Thota, A, Bansal, R, Kwon, J, Korc, M & Kota, J 2017, Methods for microRNA profiling in cancer. in Methods in Pharmacology and Toxicology. Methods in Pharmacology and Toxicology, Humana Press Inc., pp. 97-113. https://doi.org/10.1007/978-1-4939-6743-8_7
Yarlagadda S, Thota A, Bansal R, Kwon J, Korc M, Kota J. Methods for microRNA profiling in cancer. In Methods in Pharmacology and Toxicology. Humana Press Inc. 2017. p. 97-113. (Methods in Pharmacology and Toxicology). https://doi.org/10.1007/978-1-4939-6743-8_7
Yarlagadda, Sushuma ; Thota, Anusha ; Bansal, Ruchi ; Kwon, Jason ; Korc, Murray ; Kota, Janaiah. / Methods for microRNA profiling in cancer. Methods in Pharmacology and Toxicology. Humana Press Inc., 2017. pp. 97-113 (Methods in Pharmacology and Toxicology).
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