Methods for microRNA profiling in cancer

Sushuma Yarlagadda, Anusha Thota, Ruchi Bansal, Jason Kwon, Murray Korc, Janaiah Kota

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

MicroRNAs (miRNA) are small non-coding RNAs that negatively regulate post-transcriptional gene expression. Almost all human cancers are characterized by abnormal microRNA expression patterns, which are unique to tumor types. A large body of experimental evidence documents the role of miRNAs in cancer pathogenesis, and specific miRNAs function as oncogenes or tumor suppressors. Due to unique expression profiles and anti/pro-tumorigenic properties of miRNAs, efforts are underway to explore their therapeutic and diagnostic potential. Many miRNA profiling methods have been developed, ranging from Northern blotting and qRT-PCR to the more recent microarray and RNA-Seq platforms. The following chapter details an imaging technique for cellular-specific miRNA expression profiling called in situ hybridization (ISH).

Original languageEnglish (US)
Title of host publicationMethods in Pharmacology and Toxicology
PublisherHumana Press Inc.
Pages97-113
Number of pages17
DOIs
StatePublished - Jan 1 2017

Publication series

NameMethods in Pharmacology and Toxicology
ISSN (Print)1557-2153
ISSN (Electronic)1940-6053

Keywords

  • Cancer
  • Expression profiling
  • ISH (in situ hybridization)
  • Molecular imaging
  • microRNA

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Pharmacology (medical)

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