Methods for the prediction of cleavage sites of the polypeptide in certain proteins

Michael E. Miller, David M. Reboussin, Aśok C. Antony

Research output: Contribution to journalArticle

Abstract

Many proteins critical to the existence of parasites from infectious agents that affect man, including malaria, leishmania, and trypansoma, are attached to the surface membrane by glycosyl-phosphatidyl inositol (GPI) anchors. Creation of GPI-anchored proteins involves elimination of a bond between adjacent amino acids in a newly formed, or nascent, polypeptide and attachment of a GPI anchor at this site. Because interfering with this process by altering the location of attachment is a potential infection-control strategy in humans, knowledge of the cleavage site in the nascent polypeptide is important. Determination of the cleavage point directly through biochemical analysis is both labor and resource intensive. We develop both likelihood-based and Bayesian approaches for use in the identification of the site of cleavage of GPI-anchored proteins. These methods are demonstrated using four examples where extensive biochemical analyses already have identified the bond in the newly formed polypeptide where a GPI anchor is attached. In contrast to sole reliance on biochemical analyses, use of the proposed methods has great potential for savings of both time and money.

Original languageEnglish (US)
Pages (from-to)151-166
Number of pages16
JournalJournal of Agricultural, Biological, and Environmental Statistics
Volume5
Issue number2
DOIs
StatePublished - Jun 2000

Fingerprint

Polypeptides
Phosphatidylinositols
Anchors
anchor
cleavage
polypeptides
Proteins
Protein
Peptides
protein
prediction
Prediction
Malaria
proteins
malaria
Bayesian Approach
Infection
Amino Acids
Elimination
Control Strategy

Keywords

  • Bayesian analysis
  • Distance measures
  • Gamma distribution
  • GPI-anchored proteins

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Agricultural and Biological Sciences (miscellaneous)
  • Environmental Science(all)
  • Applied Mathematics
  • Statistics and Probability

Cite this

Methods for the prediction of cleavage sites of the polypeptide in certain proteins. / Miller, Michael E.; Reboussin, David M.; Antony, Aśok C.

In: Journal of Agricultural, Biological, and Environmental Statistics, Vol. 5, No. 2, 06.2000, p. 151-166.

Research output: Contribution to journalArticle

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