Methods in laboratory investigation

A simple, quantitative method for assessing angiogenesis and antiangiogenic agents using reconstituted basement membrane, heparin, and fibroblast growth factor

A. Passaniti, R. M. Taylor, Roberto Pili, Y. Guo, P. V. Long, J. A. Haney, R. R. Pauly, D. S. Grant, G. R. Martin

Research output: Contribution to journalArticle

728 Citations (Scopus)

Abstract

BACKGROUND: Blood vessel growth is necessary for normal tissue homeostasis and contributes to solid tumor growth. Methods to quantitate neovascularization should be useful in testing biological factors and drugs that regulate angiogenesis or to induce a vascular supply to promote wound healing. EXPERIMENTAL DESIGN: An extract of basement membrane proteins (Matrigel) was found to reconstitute into a gel when injected subcutaneously into C57/BL mice and to support an intense vascular response when supplemented with angiogenic factors. RESULTS: New vessels and von Willebrand factor antigen staining were apparent in the gel 2-3 days after injection, reaching a maximum after 3-5 days. Hemoglobin content of the gels was found to parallel the increase in vessels in the gel allowing ready quantitation. Angiogenesis was obtained with both acidic and basic fibroblast growth factors and was enhanced by heparin. Several substances were tested for angiostatic activity in this assay by coinjection in Matrigel with fibroblast growth factor and heparin. Platelet-derived growth factor BB, interleukin 1- β, interleukin-6, and transforming growth factor-β were potent inhibitors of neovascularization induced by fibroblast growth factor. Tumor necrosis factor-α did not alter the response but was alone a potent inducer of neovascularization when coinjected with Matrigel and heparin. Consistent with the previously demonstrated importance of collagenase in mediating endothelial cell invasion, a tissue inhibitor of metalloproteinases that also inhibits collagenases was found to be a potent inhibitor of fibroblast growth factor-induced angiogenesis. CONCLUSIONS: Our assay allows the ready quantitative assessment of angiogenic and anti-angiogenic factors and should be useful in the isolation of endothelial cells from the capillaries that penetrate into the gel.

Original languageEnglish (US)
Pages (from-to)519-528
Number of pages10
JournalLaboratory Investigation
Volume67
Issue number4
StatePublished - 1992
Externally publishedYes

Fingerprint

Angiogenesis Inhibitors
Fibroblast Growth Factors
Basement Membrane
Heparin
Gels
Blood Vessels
Angiogenesis Inducing Agents
Collagenases
Endothelial Cells
Tissue Inhibitor of Metalloproteinases
Fibroblast Growth Factor 1
von Willebrand Factor
Biological Factors
Transforming Growth Factors
Fibroblast Growth Factor 2
Growth
Interleukin-1
Wound Healing
Interleukin-6
Membrane Proteins

Keywords

  • Matrigel
  • Neovascularization

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Methods in laboratory investigation : A simple, quantitative method for assessing angiogenesis and antiangiogenic agents using reconstituted basement membrane, heparin, and fibroblast growth factor. / Passaniti, A.; Taylor, R. M.; Pili, Roberto; Guo, Y.; Long, P. V.; Haney, J. A.; Pauly, R. R.; Grant, D. S.; Martin, G. R.

In: Laboratory Investigation, Vol. 67, No. 4, 1992, p. 519-528.

Research output: Contribution to journalArticle

Passaniti, A. ; Taylor, R. M. ; Pili, Roberto ; Guo, Y. ; Long, P. V. ; Haney, J. A. ; Pauly, R. R. ; Grant, D. S. ; Martin, G. R. / Methods in laboratory investigation : A simple, quantitative method for assessing angiogenesis and antiangiogenic agents using reconstituted basement membrane, heparin, and fibroblast growth factor. In: Laboratory Investigation. 1992 ; Vol. 67, No. 4. pp. 519-528.
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AU - Passaniti, A.

AU - Taylor, R. M.

AU - Pili, Roberto

AU - Guo, Y.

AU - Long, P. V.

AU - Haney, J. A.

AU - Pauly, R. R.

AU - Grant, D. S.

AU - Martin, G. R.

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N2 - BACKGROUND: Blood vessel growth is necessary for normal tissue homeostasis and contributes to solid tumor growth. Methods to quantitate neovascularization should be useful in testing biological factors and drugs that regulate angiogenesis or to induce a vascular supply to promote wound healing. EXPERIMENTAL DESIGN: An extract of basement membrane proteins (Matrigel) was found to reconstitute into a gel when injected subcutaneously into C57/BL mice and to support an intense vascular response when supplemented with angiogenic factors. RESULTS: New vessels and von Willebrand factor antigen staining were apparent in the gel 2-3 days after injection, reaching a maximum after 3-5 days. Hemoglobin content of the gels was found to parallel the increase in vessels in the gel allowing ready quantitation. Angiogenesis was obtained with both acidic and basic fibroblast growth factors and was enhanced by heparin. Several substances were tested for angiostatic activity in this assay by coinjection in Matrigel with fibroblast growth factor and heparin. Platelet-derived growth factor BB, interleukin 1- β, interleukin-6, and transforming growth factor-β were potent inhibitors of neovascularization induced by fibroblast growth factor. Tumor necrosis factor-α did not alter the response but was alone a potent inducer of neovascularization when coinjected with Matrigel and heparin. Consistent with the previously demonstrated importance of collagenase in mediating endothelial cell invasion, a tissue inhibitor of metalloproteinases that also inhibits collagenases was found to be a potent inhibitor of fibroblast growth factor-induced angiogenesis. CONCLUSIONS: Our assay allows the ready quantitative assessment of angiogenic and anti-angiogenic factors and should be useful in the isolation of endothelial cells from the capillaries that penetrate into the gel.

AB - BACKGROUND: Blood vessel growth is necessary for normal tissue homeostasis and contributes to solid tumor growth. Methods to quantitate neovascularization should be useful in testing biological factors and drugs that regulate angiogenesis or to induce a vascular supply to promote wound healing. EXPERIMENTAL DESIGN: An extract of basement membrane proteins (Matrigel) was found to reconstitute into a gel when injected subcutaneously into C57/BL mice and to support an intense vascular response when supplemented with angiogenic factors. RESULTS: New vessels and von Willebrand factor antigen staining were apparent in the gel 2-3 days after injection, reaching a maximum after 3-5 days. Hemoglobin content of the gels was found to parallel the increase in vessels in the gel allowing ready quantitation. Angiogenesis was obtained with both acidic and basic fibroblast growth factors and was enhanced by heparin. Several substances were tested for angiostatic activity in this assay by coinjection in Matrigel with fibroblast growth factor and heparin. Platelet-derived growth factor BB, interleukin 1- β, interleukin-6, and transforming growth factor-β were potent inhibitors of neovascularization induced by fibroblast growth factor. Tumor necrosis factor-α did not alter the response but was alone a potent inducer of neovascularization when coinjected with Matrigel and heparin. Consistent with the previously demonstrated importance of collagenase in mediating endothelial cell invasion, a tissue inhibitor of metalloproteinases that also inhibits collagenases was found to be a potent inhibitor of fibroblast growth factor-induced angiogenesis. CONCLUSIONS: Our assay allows the ready quantitative assessment of angiogenic and anti-angiogenic factors and should be useful in the isolation of endothelial cells from the capillaries that penetrate into the gel.

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